Subtractive imaging in confocal scanning microscopy using a CCD camera as a detector

We report a scheme for the detector system of confocal microscopes in which the pinhole and a large-area detector are substituted by a CCD camera. The numerical integration of the intensities acquired by the active pixels emulates the signal passing through the pinhole. We demonstrate the imaging ca...

Full description

Saved in:
Bibliographic Details
Published in:Optics letters 2012-04, Vol.37 (7), p.1280-1282
Main Authors: Sánchez-Ortiga, Emilio, Sheppard, Colin J R, Saavedra, Genaro, Martínez-Corral, Manuel, Doblas, Ana, Calatayud, Arnau
Format: Article
Language:English
Subjects:
Algorithms
CCD cameras
Confocal
Detectors
Equipment Design
Image Processing, Computer-Assisted
Imaging
Microscopes
Microscopy
Microscopy, Confocal - instrumentation
Microscopy, Confocal - methods
Onions - ultrastructure
Optical sectioning
Pinholes
Subtraction Technique
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We report a scheme for the detector system of confocal microscopes in which the pinhole and a large-area detector are substituted by a CCD camera. The numerical integration of the intensities acquired by the active pixels emulates the signal passing through the pinhole. We demonstrate the imaging capability and the optical sectioning of the system. Subtractive-imaging confocal microscopy can be implemented in a simple manner, providing superresolution and improving optical sectioning.
ISSN:0146-9592
1539-4794
DOI:10.1364/ol.37.001280