Campylobacter spp. distribution in biofilms on different surfaces in an agricultural watershed (Elk Creek, British Columbia): Using biofilms to monitor for Campylobacter

• Published in: International journal of hygiene and environmental health 2012-04, Vol.215 (3), p.270-278
• Main Authors: Maal-Bared, Rasha, Bartlett, Karen H., Bowie, William R., Hall, Eric R.
• Format: Article
• Language: English
• Subjects: Air. Soil. Water. Waste. Feeding; Biofilms; Biological and medical sciences; British Columbia; Campylobacter - isolation & purification; Campylobacter - physiology; Campylobacter spp; Enterococci; Environment. Living conditions; Environmental Monitoring; Geologic Sediments - microbiology; Indicators; Medical sciences; Miscellaneous; Nitrates; Public health. Hygiene; Public health. Hygiene-occupational medicine; Surface Properties; Toxicology; Water Microbiology; Water Quality; Water Supply
• ISSN: 1438-4639; 1618-131X
• DOI: 10.1016/j.ijheh.2011.12.004

Despite its relevance to public health, presence and concentrations of Campylobacter spp. in biofilms in natural aquatic environments has not been investigated. This study examined the occurrence of Campylobacter spp. in biofilms on a variety of surfaces (river rock, slate rock, wood, Lexan™, sandpaper, and sediment) and in water from December 2005 to December 2006 to find a substratum that facilitated campylobacters detection in natural aquatic environments. Samples were collected at four sites in an agricultural watershed (Elk Creek, British Columbia). Campylobacter spp. presence was determined using culturing methods. Correlations between chemical, physical and microbiological water quality parameters and Campylobacter spp. distribution on different surface types were also investigated. Campylobacter spp. had a prevalence of 13% in the wet season, but was not recovered in the dry season. Its prevalence was highest in sediment (27%), followed by slate rock (22%), Lexan and wood (13%), river rock (9%) and water (8%), respectively. No Campylobacter spp. was found in sandpaper biofilms. Several other criteria were used to assess substrata effectiveness, such as correlation amongst Campylobacter spp., indicator bacteria and water quality parameters, cost and availability of substratum, potential for standardizing substratum, ease of biofilm removal and probability of substratum loss in situ. Results show that sediment, slate rock or wood could be used as substrata for Campylobacter spp. monitoring. The study also highlights the potential use of nitrates and enterococci as faecal contamination indicators to protect public health.