Cloning and sequence analysis of IL-2, IL-4 and IFN-γ from Indian Dromedary camels (Camelus dromedarius)

• Published in: Research in veterinary science 2012-06, Vol.92 (3), p.420-426
• Main Authors: Nagarajan, G., Swami, Shelesh Kumar, Ghorui, S.K., Pathak, K.M.L., Singh, R.K., Patil, N.V.
• Format: Article
• Language: English
• Subjects: Amino Acid Sequence; Animals; Camelus - genetics; Camelus - immunology; Cloning, Molecular; Cytokine genes; Dromedary camel; Interferon-gamma - chemistry; Interferon-gamma - genetics; Interferon-gamma - metabolism; Interleukin-2 - chemistry; Interleukin-2 - genetics; Interleukin-2 - metabolism; Interleukin-4 - chemistry; Interleukin-4 - genetics; Interleukin-4 - metabolism; Leukocytes, Mononuclear - metabolism; Male; Molecular Sequence Data; Phylogenetic analysis; Phylogeny
• ISSN: 0034-5288; 1532-2661
• DOI: 10.1016/j.rvsc.2011.03.028

The cDNAs of three cytokines, viz., IL-2, IL-4 and IFN-γ from Dromedary camels were amplified by PCR using Bactrian camel sequences and subsequently cloned for sequence analysis. Relationship based on amino acid sequences revealed that Dromedary camel IL-2 shared 99.5% and 99.3% identity at the nucleotide and amino acid levels with Bactrian camel IL-2. In the case of IL-4, the identity of Dromedary camel was 99.7% and 99.2% at the nucleotide and amino acid levels, respectively with that of Bactrian camel. The Dromedary camel IFN-γ shared 100% identity both at nucleotide and amino acid levels with Bactrian camel IFN-γ. Phylogenetic analysis based on amino acid sequences indicated the close relationship in these cytokine genes between the Dromedary camel and other camelids.