Antibiotic resistance of Escherichia coli isolated from a poultry slaughterhouse

The aim of the study was to investigate the antibiotic resistant E. coli strains isolated from bioaerosols and surface swabs in a slaughterhouse as a possible source of poultry meat contamination. The highest air coliforms contamination was during shackling, killing and evisceration of poultry. The...

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Bibliographic Details
Published in:Annals of Agricultural and Environmental Medicine 2012, Vol.19 (1), p.75-77
Main Authors: Gregova, Gabriela, Kmetova, Marta, Kmet, Vladimfr, Venglovsky, Jan, Feher, Alexander
Format: Article
Language:English
Subjects:
Abattoirs
Aerosols
Air Microbiology
Air Pollution, Indoor - analysis
Airborne microorganisms
Ampicillin
Animals
Anti-Bacterial Agents - pharmacology
Antibiotic resistance
Antibiotics
Ceftazidime
Chloramphenicol
Coliforms
Colony Count, Microbial
Contamination
Drug Resistance, Bacterial
E coli
Enrofloxacin
Enterobacteriaceae - classification
Enterobacteriaceae - isolation & purification
Escherichia coli
Escherichia coli - classification
Escherichia coli - drug effects
Escherichia coli - isolation & purification
Florfenicol
Food contamination
Food Microbiology
Gentamicin
Meat
Meat - microbiology
Microbial Sensitivity Tests - veterinary
Minimum inhibitory concentration
Polymerase chain reaction
Poultry
Poultry - microbiology
Slovakia
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - veterinary
Streptomycin
Sulbactam
Tetracyclines
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Summary:The aim of the study was to investigate the antibiotic resistant E. coli strains isolated from bioaerosols and surface swabs in a slaughterhouse as a possible source of poultry meat contamination. The highest air coliforms contamination was during shackling, killing and evisceration of poultry. The strains showed resistance to ampicillin (89%), ceftiofur (62%) and cefquinome (22%), while resistance to ampicillin with sulbactam was only 6%. Resistance to streptomycin and gentamicin was detected in 43% vs. 14% isolates; to tetracycline 33%; to chloramphenicol and florfenicol in 10% vs. 18% isolates; to cotrimoxazol in 35% isolates; to enrofloxacin in 43% isolates. The higher MIC of ceftazidime (3.6 mg x l(-1)) and ceftriaxon (5.2 mg x l(-1)) revealed the presence of ESBLs in 43% of isolates. From 19 selected phenotypically ESBL positive strains, 16 consisted of CMY-2 genes, while CTX-M genes were not detected by PCR. Maldi tof analysis of selected E. coli showed a clear clonal relatedness of environmental strains from various withdrawals.
ISSN:1232-1966
1898-2263