Differential scanning calorimetry study of blood serum in chronic obstructive pulmonary disease

• Published in: Journal of thermal analysis and calorimetry 2010-10, Vol.102 (1), p.57-60
• Main Authors: Michnik, A., Drzazga, Z., Michalik, K., Barczyk, A., Santura, I., Sozańska, E., Pierzchała, W.
• Format: Article
• Language: English
• Subjects: Analytical Chemistry; Biological and medical sciences; Biological products; Blood; Calorimetry; Care and treatment; Chemistry; Chemistry and Materials Science; Corticosteroids; Denaturation; Differential scanning calorimetry; Endothermic reactions; Inorganic Chemistry; Investigative techniques, diagnostic techniques (general aspects); Lung diseases, Obstructive; Measurement Science and Instrumentation; Medical research; Medical sciences; Medicine, Experimental; Miscellaneous. Technology; Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques; Patients; Physical Chemistry; Polymer Sciences; Proteins; Serums
• ISSN: 1388-6150; 1588-2926; 1572-8943
• DOI: 10.1007/s10973-009-0602-6

Differential scanning calorimetry (DSC) has been applied for studies of blood serum from patients sick with chronic obstructive pulmonary disease (COPD). The denaturation of serum proceeds as endothermic process over the temperature range 45–85 °C. Distinct changes in the shape of DSC curves have been observed for serum from patients with severe stage of COPD (treated with inhaled corticosteroids) relative to serum from healthy individuals. The first moment of the thermal transition with respect to the temperature axis shifts from the normal value of 63.9 ± 0.3 to 65.3 ± 0.7 °C and to 67.6 ± 1.6 °C for early and advanced stages of disease, respectively. The results of our studies suggest age dependence of blood serum denaturation transition.