Optically trapped microsensors for microfluidic temperature measurement by fluorescence lifetime imaging microscopy

The novel combination of optical tweezers and fluorescence lifetime imaging microscopy (FLIM) has been used, in conjunction with specially developed temperature-sensitive fluorescent microprobes, for the non-invasive measurement of temperature in a microfluidic device. This approach retains the capa...

Full description

Saved in:
Bibliographic Details
Published in:Lab on a chip 2011-01, Vol.11 (22), p.3821-3828
Main Authors: Bennet, Mathieu A, Richardson, Patricia R, Arlt, Jochen, McCarthy, Aongus, Buller, Gerald S, Jones, Anita C
Format: Article
Language:English
Subjects:
Droplets
Dyes
Fluorescence
Imaging
Microfluidics
Microscopy
Refractive index
Shells
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The novel combination of optical tweezers and fluorescence lifetime imaging microscopy (FLIM) has been used, in conjunction with specially developed temperature-sensitive fluorescent microprobes, for the non-invasive measurement of temperature in a microfluidic device. This approach retains the capability of FLIM to deliver quantitative mapping of microfluidic temperature without the disadvantageous need to introduce a fluorescent dye that pervades the entire micofluidic system. This is achieved by encapsulating the temperature-sensitive Rhodamine B fluorophore within a microdroplet which can be held and manipulated in the microfluidic flow using optical tweezers. The microdroplet is a double bubble in which an aqueous droplet of the fluorescent dye is surrounded by an oil shell which serves both to contain the fluorophore and to provide the refractive index differential required for optical trapping of the droplet in an external aqueous medium.
ISSN:1473-0197
1473-0189
DOI:10.1039/c1lc20391f