Preparation of heat-induced artificial collagen gels based on collagen-mimetic dendrimers

Collagen, a major component of the extracellular matrix, contains Gly-Pro-Hyp repeats that form a hydrogel. In this study, artificial collagen-mimetic materials were designed. Synthetic dendritic macromolecules were fully modified with (Pro-Hyp-Gly)n and named collagen-mimetic dendrimers. A collagen...

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Bibliographic Details
Published in:Soft matter 2011-01, Vol.7 (19), p.8991-8997
Main Authors: Kojima, Chie, Suehiro, Tomoyuki, Tada, Tomoko, Sakamoto, Yasuhiro, Waku, Tomonori, Tanaka, Naoki
Format: Article
Language:English
Subjects:
Collagens
Dendrimers
Dichroism
Heating
Hydrogels
Macromolecules
Peptides
Sol gel process
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Summary:Collagen, a major component of the extracellular matrix, contains Gly-Pro-Hyp repeats that form a hydrogel. In this study, artificial collagen-mimetic materials were designed. Synthetic dendritic macromolecules were fully modified with (Pro-Hyp-Gly)n and named collagen-mimetic dendrimers. A collagen-like triple helical structure was observed by circular dichroism spectrometry, with an efficiency that depended on the peptide length. A (Pro-Hyp-Gly)10-modified dendrimer exhibited the most efficient triple helix formation. Thermal stability was enhanced by clustering at the surface of the dendrimer. The (Pro-Hyp-Gly)10-modified dendrimer was assembled by heating and the assembly was affected by temperature, time and concentration. Hydrogels based on the (Pro-Hyp-Gly)10-modified dendrimer, but not on the peptide itself, were successfully prepared by heating. The sol-gel transition behavior was similar to natural collagen but not gelatin, which is thermally denatured collagen. Dynamic rheological analysis showed that the sol-gel transition temperature and the strength depended on the concentration. Thus, the collagen-mimetic dendrimer incorporating (Pro-Hyp-Gly)10 is an injectable and controllable artificial collagen gel.
ISSN:1744-683X
1744-6848
DOI:10.1039/c1sm06157g