Nanopatterned Cadmium Selenide Langmuir–Blodgett Platform for Leukemia Detection

We present results of the studies relating to preparation of Langmuir–Blodgett (LB) monolayers of tri-n-octylphosphine oxide-capped cadmium selenide quantum dots (QCdSe) onto indium–tin oxide (ITO) coated glass substrate. The monolayer behavior has been studied at the air–water interface under vario...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2012-04, Vol.84 (7), p.3082-3089
Main Authors: Sharma, Aditya, Pandey, Chandra M, Matharu, Zimple, Soni, Udit, Sapra, Sameer, Sumana, Gajjala, Pandey, Manoj K, Chatterjee, Tathagat, Malhotra, Bansi D
Format: Article
Language:English
Subjects:
Analytical chemistry
Base Sequence
Biological and medical sciences
Biosensing Techniques - instrumentation
Biosensing Techniques - methods
Biosensors
Biotechnology
Cadmium Compounds - chemistry
Cadmium selenide
Chemistry
DNA Probes - chemistry
DNA Probes - genetics
Electrocatalysis
Electrochemical methods
Electrochemistry
Electrodes
Equipment Reuse
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
General, instrumentation
Humans
Indium tin oxides
Leukemia
Leukemia, Myelogenous, Chronic, BCR-ABL Positive - diagnosis
Methods. Procedures. Technologies
Nanotechnology - instrumentation
Nanotechnology - methods
Nucleic Acid Hybridization
Organophosphorus Compounds - chemistry
Quantum Dots
Selenium Compounds - chemistry
Surface Properties
Tin Compounds - chemistry
Various methods and equipments
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Summary:We present results of the studies relating to preparation of Langmuir–Blodgett (LB) monolayers of tri-n-octylphosphine oxide-capped cadmium selenide quantum dots (QCdSe) onto indium–tin oxide (ITO) coated glass substrate. The monolayer behavior has been studied at the air–water interface under various subphase conditions. This nanopatterned platform has been explored to fabricate an electrochemical DNA biosensor for detection of chronic myelogenous leukemia (CML) by covalently immobilizing the thiol-terminated oligonucleotide probe sequence via a displacement reaction. The results of electrochemical response studies reveal that this biosensor can detect target DNA in the range of 10–6 to 10–14 M within 120 s, has a shelf life of 2 months, and can be used about 8 times. Further, this nucleic acid sensor has been found to distinguish the CML-positive and the control negative clinical patient samples.
ISSN:0003-2700
1520-6882
DOI:10.1021/ac202265a