novel nuclear marker, Pm-int9, for phylogenetic studies of Opisthorchis felineus, Opisthorchis viverrini, and Clonorchis sinensis (Opisthorchiidae, Trematoda)

Opisthorchis felineus, O. viverrini, and Clonorchis sinensis, the trematodes of the family Opisthorchiidae, are important human parasites. Two previous studies (Kang et al. Parasitol Int 57:191-197, 2008; Katokhin et al. Dokl Biochem Biophys 421:214-217, 2008) have provided evidence using ribosomal...

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Published in:Parasitology research (1987) 2009-12, Vol.106 (1), p.293-297
Main Authors: Shekhovtsov, Sergei V, Katokhin, Alexei V, Romanov, Konstantin V, Besprozvannykh, Vladimir V, Fedorov, Konstantin P, Yurlova, Natalia I, Serbina, Elena A, Sithithaworn, Paiboon, Kolchanov, Nikolai A, Mordvinov, Viatcheslav A
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Biomedical and Life Sciences
Biomedicine
Clonorchiasis - parasitology
Clonorchiasis - veterinary
Clonorchis sinensis
Clonorchis sinensis - classification
Clonorchis sinensis - genetics
Clonorchis sinensis - isolation & purification
Genetic Markers
Genotype
Helminth Proteins - genetics
Immunology
Medical Microbiology
Microbiology
Molecular Sequence Data
Opisthorchiasis - parasitology
Opisthorchiasis - veterinary
Opisthorchiidae
Opisthorchis
Opisthorchis - classification
Opisthorchis - genetics
Opisthorchis - isolation & purification
Opisthorchis felineus
Opisthorchis viverrini
Phylogeny
Polymorphism, Genetic
Sequence Alignment
Sequence Analysis, DNA
Short Communication
Siberia
Thailand
Trematoda
Tropomyosin - genetics
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Summary:Opisthorchis felineus, O. viverrini, and Clonorchis sinensis, the trematodes of the family Opisthorchiidae, are important human parasites. Two previous studies (Kang et al. Parasitol Int 57:191-197, 2008; Katokhin et al. Dokl Biochem Biophys 421:214-217, 2008) have provided evidence using ribosomal and mitochondrial sequences that O. viverrini, O. felineus, and C. sinensis are closely related. We developed a novel nuclear marker, Pm-int9, which included the ninth intron of the paramyosin gene and flanking exon sequences. Samples of O. felineus from four localities of West Siberia, C. sinensis from the Russian Far East, and O. viverrini from Thailand were genotyped by Pm-int9. Little variation was detected in exon sequences, however, intron sequences turned out to be more variable than ribosomal internal transcribed spacers. We can conclude that Pm-int9 is valuable for interspecific variation studies. Phylogenetic analysis based on Pm-int9 revealed that O. viverrini and C. sinensis were closer to each other than either of them to O. felineus, supporting the opinion that C. sinensis should be considered the sister species of Opisthorchis spp.
ISSN:0932-0113
1432-1955
DOI:10.1007/s00436-009-1628-1