Phosphate Transporter Promoter from Arabidopsis thaliana AtPHT1;4 Gene Drives Preferential Gene Expression in Transgenic Maize Roots Under Phosphorus Starvation

Phosphorus (P) stress responsive genes have been identified and characterized, including the high-affinity phosphate transporter AtPHT1;4 from Arabidopsis thaliana. This gene encodes a membrane protein that is primarily expressed in roots under phosphorus deficiency. A 2.3-kb promoter region from At...

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Bibliographic Details
Published in:Plant molecular biology reporter 2010-12, Vol.28 (4), p.717-723
Main Authors: Coelho, Gracielle T. C. P, Carneiro, Newton P, Karthikeyan, Athikkattuvalasu S, Raghothama, Kaschandra G, Schaffert, Robert E, Brandão, Rosangela L, Paiva, Luciano V, Souza, Isabel R. P, Alves, Vera M, Imolesi, Anderson, Carvalho, Carlos H. S, Carneiro, Andréa A
Format: Article
Language:English
Subjects:
active transport
Arabidopsis thaliana
Bioinformatics
Biomedical and Life Sciences
Bombardment
Corn
Gene expression
gene expression regulation
genes
leaves
Life Sciences
Membrane proteins
Metabolomics
molecular sequence data
nutrient deficiencies
nutrient uptake
Phosphate starvation response
Phosphate transporter
phosphates
Phosphorus
Plant Breeding/Biotechnology
plant nutrition
Plant Sciences
Plants (botany)
promoter regions
Proteomics
Reporter gene
reporter genes
Rhizosphere
Roots
Stresses
Transgenic plants
transport proteins
Zea mays
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Summary:Phosphorus (P) stress responsive genes have been identified and characterized, including the high-affinity phosphate transporter AtPHT1;4 from Arabidopsis thaliana. This gene encodes a membrane protein that is primarily expressed in roots under phosphorus deficiency. A 2.3-kb promoter region from AtPHT1;4 has been fused with the β-glucuronidase (GUS) encoding gene and introduced into maize via biolistic bombardment to evaluate its spatiotemporal activity in a heterologous system. AtPHT1;4::GUS expression is detected preferentially in transgenic maize roots under P deficiency. Further analysis of transgenic plants has also revealed that GUS activity is higher in roots than in leaves by about sixfold. These results demonstrate the ability of AtPHT1;4 promoter to direct expression of the reporter gene in a monocot root system under P stress. This property of AtPHT1;4 promoter makes it useful to engineer maize plants to modify the soil's rhizosphere and increase efficiency of P acquisition under P stress conditions.
ISSN:0735-9640
1572-9818
DOI:10.1007/s11105-010-0199-8