Diverse LEA (late embryogenesis abundant) and LEA-like genes and their responses to hypersaline stress in post-diapause embryonic development of Artemia franciscana

From post-diapause cysts of Artemia franciscana, we defined fourteen LEA (late embryogenesis abundant) and LEA-like genes, including four novel members (Afrlea1-5, Afrlea3-5, Afrlea3-like1 and Afrlea3-like2), which were classified into four groups: G1, G3, G3-like (LEA group3-like), and SMP-like (se...

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Bibliographic Details
Published in:Comparative Biochemistry and Physiology Part B: Biochemistry and Molecular Biology 2011-09, Vol.160 (1), p.32-39
Main Authors: Wu, Gang, Zhang, Hangxiao, Sun, Jing, Liu, Fei, Ge, Xiaomeng, Chen, Wei-Hua, Yu, Jun, Wang, Weiwei
Format: Article
Language:English
Subjects:
amino acid composition
amino acid motifs
Amino Acid Sequence
Animals
Artemia - embryology
Artemia - genetics
Artemia - physiology
Artemia franciscana
bacteria
embryogenesis
Gene Expression Regulation
genes
Hydrophilic protein
Hypersaline stress
larvae
Late embryogenesis abundant
Life Cycle Stages - physiology
Molecular Sequence Data
Phylogeny
Plant Proteins - genetics
Plant Proteins - metabolism
Post-diapause embryos
Proteins - genetics
Proteins - metabolism
reverse transcriptase polymerase chain reaction
Salinity
salt stress
Sequence Alignment
Stress, Physiological
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Summary:From post-diapause cysts of Artemia franciscana, we defined fourteen LEA (late embryogenesis abundant) and LEA-like genes, including four novel members (Afrlea1-5, Afrlea3-5, Afrlea3-like1 and Afrlea3-like2), which were classified into four groups: G1, G3, G3-like (LEA group3-like), and SMP-like (seed-maturation-protein-like), based on their conserved and diversified sequence motifs and amino acid compositions among bacteria, plants, and animals. We also validated six representative genes based on quantitative RT-PCR, including three LEA and two LEA3-like genes that are down-regulated when dehydrated cysts hatch to desiccation-intolerant larvae as well as one SMP-like gene that is slightly up-regulated. We further tested their responses to hypersaline stress for four representatives—one from each group—and found that the expression of Afrlea1-5 and Afrlea3-2 were inducible but not Afrlea3-like1 and Afrsmp-like. This result suggested that the LEA and LEA-like genes may play different roles in resistance to hypersaline stress.
ISSN:1096-4959
1879-1107
DOI:10.1016/j.cbpb.2011.05.005