The Phosphate Transporter Gene OsPht1;8 Is Involved in Phosphate Homeostasis in Rice

• Published in: Plant physiology (Bethesda) 2011-07, Vol.156 (3), p.1164-1175
• Main Authors: Jia, Hongfang, Ren, Hongyan, Gu, Mian, Zhao, Jianning, Sun, Shubin, Zhang, Xiao, Chen, Jieyu, Wu, Ping, Xu, Guohua
• Format: Article
• Language: English
• Subjects: Animals; FOCUS ISSUE ON PHOSPHORUS PLANT PHYSIOLOGY; Gene Expression Regulation, Plant; Genes, Plant - genetics; Homeostasis - drug effects; Homeostasis - genetics; Molecular Sequence Data; Oocytes; Oocytes - drug effects; Oocytes - metabolism; Organ Specificity - drug effects; Oryza - drug effects; Oryza - genetics; Oryza - growth & development; Oryza - metabolism; Oryza sativa; Panicles; Phosphate transport proteins; Phosphate Transport Proteins - genetics; Phosphate Transport Proteins - metabolism; Phosphates; Phosphates - deficiency; Phosphates - metabolism; Phosphates - pharmacology; Plant growth; Plant Proteins - genetics; Plant Proteins - metabolism; Plant roots; Plants; Plants, Genetically Modified; Protein Transport - drug effects; Rice; Saccharomyces cerevisiae; Subcellular Fractions - drug effects; Subcellular Fractions - metabolism; Suppression, Genetic - drug effects; Transgenic plants; Xenopus; Xenopus laevis; Yeasts
• ISSN: 0032-0889; 1532-2548; 1532-2548
• DOI: 10.1104/pp.111.175240

Plant phosphate transporters (PTs) are active in the uptake of inorganic phosphate (Pi) from the soil and its translocation within the plant. Here, we report on the biological properties and physiological roles of OsPht1;8 (OsPT8), one of the PTs belonging to the Pht1 family in rice (Oryza sativa). Expression of a β-glucuronidase and green fluorescent protein reporter gene driven by the OsPT8 promoter showed that OsPT8 is expressed in various tissue organs from roots to seeds independent of Pi supply. OsPT8 was able to complement a yeast Pi-uptake mutant and increase Pi accumulation of Xenopus laevis oocytes when supplied with micromolar ³³Pi concentrations at their external solution, indicating that it has a high affinity for Pi transport. Overexpression of OsPT8 resulted in excessive Pi in both roots and shoots and Pi toxic symptoms under the high-Pi supply condition. In contrast, knockdown of OsPT8 by RNA interference decreased Pi uptake and plant growth under both high-and low-Pi conditions. Moreover, OsPT8 suppression resulted in an increase of phosphorus content in the panicle axis and in a decrease of phosphorus content in unfilled grain hulls, accompanied by lower seed-setting rate. Altogether, our data suggest that OsPT8 is involved in Pi homeostasis in rice and is critical for plant growth and development.