Production and characterization of recombinant transmembrane proteins from Mycoplasma hyopneumoniae

• Published in: Veterinary microbiology 2012-02, Vol.155 (1), p.44-52
• Main Authors: Marchioro, S.B., Simionatto, S., Galli, V., Conceição, F.R., Brum, C.B., Fisch, A., Gomes, C.K., Dellagostin, O.A.
• Format: Article
• Language: English
• Subjects: Animals; Antibodies, Bacterial - blood; Antigens, Bacterial - immunology; Bacteriology; Biological and medical sciences; Escherichia coli; Escherichia coli - genetics; Female; Fundamental and applied biological sciences. Psychology; Immunity, Humoral - immunology; Male; Membrane Proteins - genetics; Membrane Proteins - metabolism; Mice; Microbiology; Miscellaneous; Mycoplasma hyopneumoniae; Mycoplasma hyopneumoniae - genetics; Mycoplasma hyopneumoniae - metabolism; Recombinant Proteins - genetics; Recombinant Proteins - immunology; Recombinant Proteins - isolation & purification; Recombinant Proteins - metabolism; Swine enzootic pneumonia; Transmembrane proteins; Vaccines, Synthetic - immunology; Vaccines, Synthetic - metabolism
• ISSN: 0378-1135; 1873-2542
• DOI: 10.1016/j.vetmic.2011.08.001

Mycoplasma hyopneumoniae is the etiological agent of swine enzootic pneumonia (EP), a chronic respiratory disease which causes significant economic losses to the swine industry worldwide. More efficient strategies for controlling this disease are necessary. In this study, we cloned17 genes coding for transmembrane proteins from M. hyopneumoniae, among which six were successfully expressed in Escherichia coli and had their immunogenic and antigenic properties evaluated. All proteins were immunogenic in mice and sera from naturally infected pigs reacted with the recombinant proteins, suggesting that they are expressed during infection. These antigens may contribute for the development of new recombinant vaccines and diagnostic tests against EP.