Frequent PIK3CA gene amplification and its clinical significance in colorectal cancer

Using a DNA microarray approach to screen for gene copy number changes in 20 colorectal (CR) carcinoma samples and filtering for high-level DNA copy number changes, we detected an amplicon at 3q26 containing the PIK3CA gene. Fluorescence in situ hybridization was employed for evaluation of PIK3CA am...

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Bibliographic Details
Published in:The Journal of pathology 2009-11, Vol.219 (3), p.337-346
Main Authors: Jehan, Zeenath, Bavi, Prashant, Sultana, Mehar, Abubaker, Jehad, Bu, Rong, Hussain, Azhar, Alsbeih, Ghazi, Al-Sanea, Nasser, Abduljabbar, Alaa, Ashari, Luai H, Alhomoud, Samar, Al-Dayel, Fouad, Uddin, Shahab, Al-Kuraya, Khawla S
Format: Article
Language:English
Subjects:
Adenoma
Adenoma - genetics
Adenoma - metabolism
Adenoma - pathology
adjuvant therapy
Adjuvants
Adult
Age
Aged
Biological and medical sciences
Biomarkers, Tumor - genetics
Biomarkers, Tumor - metabolism
Carcinogenesis
Carcinoma
Centromeres
Chemotherapy
Chemotherapy, Adjuvant
Class I Phosphatidylinositol 3-Kinases
Colorectal cancer
Colorectal carcinoma
Colorectal Neoplasms - genetics
Colorectal Neoplasms - metabolism
Colorectal Neoplasms - pathology
Colorectal Neoplasms - therapy
copy number
DNA microarrays
Epidemiologic Methods
Female
FISH
Fluorescence in situ hybridization
Gastroenterology. Liver. Pancreas. Abdomen
Gene Amplification
Humans
In Situ Hybridization, Fluorescence
Investigative techniques, diagnostic techniques (general aspects)
Male
Medical sciences
Middle Aged
Mucosa
Mutation
Neoplasm Proteins - genetics
Neoplasm Proteins - metabolism
Oligonucleotide Array Sequence Analysis - methods
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Phosphatidylinositol 3-Kinases - genetics
Phosphatidylinositol 3-Kinases - metabolism
PIK3CA gene
Prognosis
Radiotherapy
Radiotherapy, Adjuvant
Reverse Transcriptase Polymerase Chain Reaction - methods
Stomach. Duodenum. Small intestine. Colon. Rectum. Anus
Survival
tissue microarray
Treatment Outcome
Tumors
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Description
Summary:Using a DNA microarray approach to screen for gene copy number changes in 20 colorectal (CR) carcinoma samples and filtering for high-level DNA copy number changes, we detected an amplicon at 3q26 containing the PIK3CA gene. Fluorescence in situ hybridization was employed for evaluation of PIK3CA amplification on a progression CR tissue microarray containing 448 CR carcinomas, normal mucosa, and adenomas with follow-up information. PIK3CA amplification (ratio PIK3CA/centromere 3greater-than-or-equal2.0) was found in 38% of cancers, while another 19% of tumours had PIK3CA gains (ratio >1.0 but
ISSN:0022-3417
1096-9896
DOI:10.1002/path.2601