Prophylactic effect of Lactobacillus oral vaccine expressing a Japanese cedar pollen allergen

• Published in: Journal of bioscience and bioengineering 2012-04, Vol.113 (4), p.536-541
• Main Authors: Ohkouchi, Kayo, Kawamoto, Seiji, Tatsugawa, Kenji, Yoshikawa, Noboru, Takaoka, Yuki, Miyauchi, Sayumi, Aki, Tsunehiro, Yamashita, Mitsuo, Murooka, Yoshikatsu, Ono, Kazuhisa
• Format: Article
• Language: English
• Subjects: Adjuvants; Administration, Oral; Allergen; allergens; Allergens - genetics; Allergens - immunology; animal models; Animals; Anti-Allergic Agents - immunology; Antigens, Plant - genetics; Antigens, Plant - immunology; Biological and medical sciences; Biotechnology; Codon optimization; codons; complementary DNA; Cryptomeria - genetics; Cryptomeria - immunology; Cryptomeria japonica; Fundamental and applied biological sciences. Psychology; gene fusion; genes; hay fever; heterologous gene expression; IgE; immune response; immunoglobulin E; Immunoglobulin E - blood; Japanese cedar pollen; Lactate dehydrogenase; lactic acid bacteria; Lactobacillus; Lactobacillus - genetics; Lactobacillus plantarum; Mice; Models, Animal; nose; oral vaccination; Plant Proteins - genetics; Plant Proteins - immunology; pollen; Pollen - genetics; Pollen - immunology; polymerase chain reaction; protective effect; Rhinitis, Allergic, Seasonal - prevention & control; site-directed mutagenesis; vaccines; Vaccines, DNA - administration & dosage; Vaccines, DNA - immunology
• ISSN: 1389-1723; 1347-4421
• DOI: 10.1016/j.jbiosc.2011.11.025

Lactic acid bacteria (LAB) represent an attractive delivery vehicle for oral allergy vaccine because of their safety as a food microorganism as well as their potent adjuvant activity triggering anti-allergic immune response. Here, we report the generation of recombinant LAB expressing a major Japanese cedar pollen allergen Cry j 1 (Cry j 1-LAB), and their prophylactic effect in vivo. To facilitate heterologous expression, the codon usage in the Cry j 1 gene was optimized for the host LAB strain Lactobacillus plantarum by the recursive PCR-based exhaustive site-directed mutagenesis. Use of the codon-optimized Cry j 1 cDNA and a lactate dehydrogenase gene fusion system led to a successful production of recombinant Cry j 1 in L. plantarum NCL21. We also found that oral vaccination with the Cry j 1-LAB suppressed allergen-specific IgE response and nasal symptoms in a murine model of cedar pollinosis.