Confirmation of quantitative trait loci for resistance to multiple-HG types of soybean cyst nematode (Heterodera glycines Ichinohe)

• Published in: Euphytica 2011-09, Vol.181 (1), p.101-113, Article 101
• Main Authors: Vuong, T. D., Sleper, D. A., Shannon, J. G., Wu, X., Nguyen, H. T.
• Format: Article
• Language: English
• Subjects: Agronomy. Soil science and plant productions; Biological and medical sciences; Biomedical and Life Sciences; Biotechnology; Chromosomes; Classical and quantitative genetics. Population genetics. Molecular genetics; Cloning; Fundamental and applied biological sciences. Psychology; Gene mapping; Generalities. Genetics. Plant material; Genetic aspects; Genetics and breeding of economic plants; Heterodera glycines; Life Sciences; Molecular genetics; Nematoda; Nematodes; Plant Genetics and Genomics; Plant Pathology; Plant Physiology; Plant resistance; Plant Sciences; Polymorphism; Quantitative genetics; Single nucleotide polymorphisms; Soybean; Soybeans; Varietal selection. Specialized plant breeding, plant breeding aims
• ISSN: 0014-2336; 1573-5060
• DOI: 10.1007/s10681-011-0430-1

Genetic analysis of resistance of plant introduction (PI) 438489B to soybean cyst nematode (SCN) have shown that this PI is highly resistant to many SCN HG types. However, validation of the previously detected quantitative trait loci (QTL) has not been done. In this study, 250 F 2:3 progeny of a Magellan (susceptible) × PI 438489B (resistant) cross were used for primary genetic mapping to detect putative QTL for resistance to five SCN HG types. QTL confirmation study was subsequently conducted using F 6:7 recombinant inbred lines (RILs) derived from the same cross. Simple sequence repeat (SSR) and single nucleotide polymorphism (SNP) markers were employed for molecular genotyping. Interval mapping (IM), permutation tests, cofactor selection, and composite interval mapping (CIM) were performed to identify and map QTL. Results showed that five QTL intervals were associated with resistance to either multiple- or single-HG types of SCN. Among these, two major QTL for resistance to multiple-SCN HG types were mapped to chromosomes (Chr.) 8 and 18, consistent with the known rhg1 and Rhg4 locations. The other QTL were mapped to Chr. 4. The results of our study confirmed earlier reported SCN resistance QTL in this PI. Moreover, SSR and SNP molecular markers tightly linked to these QTL can be useful for the near-isogenic lines (NILs) development aiming to fine-mapping of these QTL regions and map-based cloning of SCN resistance candidate genes.