Fis regulates the competitiveness of Pseudomonas putida on barley roots by inducing biofilm formation

• Published in: Microbiology (Society for General Microbiology) 2012-03, Vol.158 (Pt 3), p.708-720
• Main Authors: JAKOVLEVA, Julia, TEPPO, Annika, VELTS, Anna, SAUMAA, Signe, MOOR, Hanna, KIVISAAR, Maia, TERAS, Riho
• Format: Article
• Language: English
• Subjects: Bacteriology; Biofilms - growth & development; Biological and medical sciences; Enterobacteriaceae; Escherichia coli; Factor For Inversion Stimulation Protein - metabolism; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation, Bacterial; Hordeum - microbiology; Hordeum vulgare; Locomotion; Microbiology; Miscellaneous; Plant Roots - microbiology; Pseudomonas putida; Pseudomonas putida - growth & development; Virulence
• ISSN: 1350-0872; 1465-2080
• DOI: 10.1099/mic.0.053355-0

An important link between the environment and the physiological state of bacteria is the regulation of the transcription of a large number of genes by global transcription factors. One of the global regulators, Fis (factor for inversion stimulation), is well studied in Escherichia coli, but the role of this protein in pseudomonads has only been examined briefly. According to studies in Enterobacteriaceae, Fis regulates positively the flagellar movement of bacteria. In pseudomonads, flagellar movement is an important trait for the colonization of plant roots. Therefore we were interested in the role of the Fis protein in Pseudomonas putida, especially the possible regulation of the colonization of plant roots. We observed that Fis reduced the migration of P. putida onto the apices of barley roots and thereby the competitiveness of bacteria on the roots. Moreover, we observed that overexpression of Fis drastically reduced swimming motility and facilitated P. putida biofilm formation, which could be the reason for the decreased migration of bacteria onto the root apices. It is possible that the elevated expression of Fis is important in the adaptation of P. putida during colonization of plant roots by promoting biofilm formation when the migration of bacteria is no longer favoured.