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Extracellular protease in Actinomycetes culture supernatants inhibits and detaches Staphylococcus aureus biofilm formation

Bacterial biofilms are associated with chronic infections due to their resistance to antimicrobial agents. Staphylococcus aureus is a versatile human pathogen and can form biofilms on human tissues and diverse medical devices. To identify novel biofilm inhibitors of S. aureus, the supernatants from...

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Published in:	Biotechnology letters 2012-04, Vol.34 (4), p.655-661
Main Authors:	Park, Joo-Hyeon, Lee, Jin-Hyung, Kim, Chang-Jin, Lee, Jae-Chan, Cho, Moo Hwan, Lee, Jintae
Format:	Article
Language:	English
Subjects:	Actinobacteria - enzymology Actinomycetales Actinomycetes Anti-Bacterial Agents - metabolism antibiotic resistance Antimicrobial agents Bacteria Bacterial Adhesion - drug effects Bioengineering biofilm Biofilms Biofilms - drug effects Biofilms - growth & development Biological and medical sciences Biotechnology Cellular biology Culture Fundamental and applied biological sciences. Psychology Humans Inhibitors Medical equipment pathogens Peptide Hydrolases - metabolism Protease Proteases proteinases Staphylococcus aureus Staphylococcus aureus - drug effects Staphylococcus aureus - physiology Staphylococcus infections Strain
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creator	Park, Joo-Hyeon Lee, Jin-Hyung Kim, Chang-Jin Lee, Jae-Chan Cho, Moo Hwan Lee, Jintae
description	Bacterial biofilms are associated with chronic infections due to their resistance to antimicrobial agents. Staphylococcus aureus is a versatile human pathogen and can form biofilms on human tissues and diverse medical devices. To identify novel biofilm inhibitors of S. aureus, the supernatants from a library of 458 Actinomycetes strains were screened. The culture supernatants (1% v/v) of more than 10 Actinomycetes strains inhibited S. aureus biofilm formation by more than 80% without affecting the growth. The culture supernatants of these biofilm-reducing Actinomycetes strains contained a protease (equivalent to 0.1 μg proteinase K ml−1), which both inhibited S. aureus biofilm formation and detached pre-existing S. aureus biofilms. This study suggests that protease treatment could be a feasible tool to reduce and eradicate S. aureus biofilms.
doi_str_mv	10.1007/s10529-011-0825-z
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Staphylococcus aureus is a versatile human pathogen and can form biofilms on human tissues and diverse medical devices. To identify novel biofilm inhibitors of S. aureus, the supernatants from a library of 458 Actinomycetes strains were screened. The culture supernatants (1% v/v) of more than 10 Actinomycetes strains inhibited S. aureus biofilm formation by more than 80% without affecting the growth. The culture supernatants of these biofilm-reducing Actinomycetes strains contained a protease (equivalent to 0.1 μg proteinase K ml−1), which both inhibited S. aureus biofilm formation and detached pre-existing S. aureus biofilms. This study suggests that protease treatment could be a feasible tool to reduce and eradicate S. aureus biofilms.</description><identifier>ISSN: 0141-5492</identifier><identifier>EISSN: 1573-6776</identifier><identifier>DOI: 10.1007/s10529-011-0825-z</identifier><identifier>PMID: 22160331</identifier><identifier>CODEN: BILED3</identifier><language>eng</language><publisher>Heidelberg: Springer-Verlag</publisher><subject>Actinobacteria - enzymology ; Actinomycetales ; Actinomycetes ; Anti-Bacterial Agents - metabolism ; antibiotic resistance ; Antimicrobial agents ; Bacteria ; Bacterial Adhesion - drug effects ; Bioengineering ; biofilm ; Biofilms ; Biofilms - drug effects ; Biofilms - growth & development ; Biological and medical sciences ; Biotechnology ; Cellular biology ; Culture ; Fundamental and applied biological sciences. Psychology ; Humans ; Inhibitors ; Medical equipment ; pathogens ; Peptide Hydrolases - metabolism ; Protease ; Proteases ; proteinases ; Staphylococcus aureus ; Staphylococcus aureus - drug effects ; Staphylococcus aureus - physiology ; Staphylococcus infections ; Strain</subject><ispartof>Biotechnology letters, 2012-04, Vol.34 (4), p.655-661</ispartof><rights>2015 INIST-CNRS</rights><rights>Springer Science+Business Media B.V. 2012</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c470t-69125656cf1d8b1240c9e33d04834ad74bda386442ba1404fda5054c167906e73</citedby><cites>FETCH-LOGICAL-c470t-69125656cf1d8b1240c9e33d04834ad74bda386442ba1404fda5054c167906e73</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=25651835$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/22160331$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Park, Joo-Hyeon</creatorcontrib><creatorcontrib>Lee, Jin-Hyung</creatorcontrib><creatorcontrib>Kim, Chang-Jin</creatorcontrib><creatorcontrib>Lee, Jae-Chan</creatorcontrib><creatorcontrib>Cho, Moo Hwan</creatorcontrib><creatorcontrib>Lee, Jintae</creatorcontrib><title>Extracellular protease in Actinomycetes culture supernatants inhibits and detaches Staphylococcus aureus biofilm formation</title><title>Biotechnology letters</title><addtitle>Biotechnol Lett</addtitle><description>Bacterial biofilms are associated with chronic infections due to their resistance to antimicrobial agents. Staphylococcus aureus is a versatile human pathogen and can form biofilms on human tissues and diverse medical devices. To identify novel biofilm inhibitors of S. aureus, the supernatants from a library of 458 Actinomycetes strains were screened. The culture supernatants (1% v/v) of more than 10 Actinomycetes strains inhibited S. aureus biofilm formation by more than 80% without affecting the growth. The culture supernatants of these biofilm-reducing Actinomycetes strains contained a protease (equivalent to 0.1 μg proteinase K ml−1), which both inhibited S. aureus biofilm formation and detached pre-existing S. aureus biofilms. This study suggests that protease treatment could be a feasible tool to reduce and eradicate S. aureus biofilms.</description><subject>Actinobacteria - enzymology</subject><subject>Actinomycetales</subject><subject>Actinomycetes</subject><subject>Anti-Bacterial Agents - metabolism</subject><subject>antibiotic resistance</subject><subject>Antimicrobial agents</subject><subject>Bacteria</subject><subject>Bacterial Adhesion - drug effects</subject><subject>Bioengineering</subject><subject>biofilm</subject><subject>Biofilms</subject><subject>Biofilms - drug effects</subject><subject>Biofilms - growth & development</subject><subject>Biological and medical sciences</subject><subject>Biotechnology</subject><subject>Cellular biology</subject><subject>Culture</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Humans</subject><subject>Inhibitors</subject><subject>Medical equipment</subject><subject>pathogens</subject><subject>Peptide Hydrolases - metabolism</subject><subject>Protease</subject><subject>Proteases</subject><subject>proteinases</subject><subject>Staphylococcus aureus</subject><subject>Staphylococcus aureus - drug effects</subject><subject>Staphylococcus aureus - physiology</subject><subject>Staphylococcus infections</subject><subject>Strain</subject><issn>0141-5492</issn><issn>1573-6776</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2012</creationdate><recordtype>article</recordtype><recordid>eNqF0k1v1DAQBuAIgehS-AFcIEJCcAnM-DM5VlVbkCpxKD1HE8dhXSXxYjtSd3893u4CEodysiU_M5b9TlG8RviEAPpzRJCsqQCxgprJavekWKHUvFJaq6fFClBgJUXDTooXMd4BQKNBPy9OGEMFnOOq2F3cp0DGjuMyUig3wSdL0ZZuLs9McrOftsYmG0uzjGkJtozLxoaZEs0pZrV2ncsbmvuyt4nMOtObRJv1dvTGG7Pks1yWl875wY1TOfgwUXJ-flk8G2iM9tVxPS1uLy--n3-prr9dfT0_u66M0JAq1SCTSiozYF93yASYxnLeg6i5oF6LrideKyFYRyhADD1JkMKg0g0oq_lp8eHQNz_u52JjaicX9y-m2folto2qUQuU_P-S6ZrzGjDLj49KVLWsQTG1p-_-oXd-yT84PvQDDc3DzXhAJvgYgx3aTXAThW2L0O6zbg9Ztznrdp91u8s1b46Nl26y_Z-K3-Fm8P4IKBoah0CzcfGvy7-KNZfZvT24gXxLP0I2tzcsD08eGKEA1KOC5VlT_Bcv0MSi</recordid><startdate>20120401</startdate><enddate>20120401</enddate><creator>Park, Joo-Hyeon</creator><creator>Lee, Jin-Hyung</creator><creator>Kim, Chang-Jin</creator><creator>Lee, Jae-Chan</creator><creator>Cho, Moo Hwan</creator><creator>Lee, Jintae</creator><general>Springer-Verlag</general><general>Springer</general><general>Springer Nature B.V</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>3V.</scope><scope>7QL</scope><scope>7QR</scope><scope>7T7</scope><scope>7TB</scope><scope>7U5</scope><scope>7X7</scope><scope>7XB</scope><scope>88A</scope><scope>88E</scope><scope>88I</scope><scope>8AO</scope><scope>8FD</scope><scope>8FE</scope><scope>8FG</scope><scope>8FH</scope><scope>8FI</scope><scope>8FJ</scope><scope>8FK</scope><scope>ABJCF</scope><scope>ABUWG</scope><scope>AFKRA</scope><scope>AZQEC</scope><scope>BBNVY</scope><scope>BENPR</scope><scope>BGLVJ</scope><scope>BHPHI</scope><scope>C1K</scope><scope>CCPQU</scope><scope>DWQXO</scope><scope>FR3</scope><scope>FYUFA</scope><scope>GHDGH</scope><scope>GNUQQ</scope><scope>HCIFZ</scope><scope>K9.</scope><scope>L6V</scope><scope>L7M</scope><scope>LK8</scope><scope>M0S</scope><scope>M1P</scope><scope>M2P</scope><scope>M7N</scope><scope>M7P</scope><scope>M7S</scope><scope>P64</scope><scope>PQEST</scope><scope>PQQKQ</scope><scope>PQUKI</scope><scope>PTHSS</scope><scope>Q9U</scope><scope>7X8</scope><scope>7QO</scope></search><sort><creationdate>20120401</creationdate><title>Extracellular protease in Actinomycetes culture supernatants inhibits and detaches Staphylococcus aureus biofilm formation</title><author>Park, Joo-Hyeon ; Lee, Jin-Hyung ; Kim, Chang-Jin ; Lee, Jae-Chan ; Cho, Moo Hwan ; Lee, Jintae</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c470t-69125656cf1d8b1240c9e33d04834ad74bda386442ba1404fda5054c167906e73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2012</creationdate><topic>Actinobacteria - enzymology</topic><topic>Actinomycetales</topic><topic>Actinomycetes</topic><topic>Anti-Bacterial Agents - metabolism</topic><topic>antibiotic resistance</topic><topic>Antimicrobial agents</topic><topic>Bacteria</topic><topic>Bacterial Adhesion - drug effects</topic><topic>Bioengineering</topic><topic>biofilm</topic><topic>Biofilms</topic><topic>Biofilms - drug effects</topic><topic>Biofilms - growth & development</topic><topic>Biological and medical sciences</topic><topic>Biotechnology</topic><topic>Cellular biology</topic><topic>Culture</topic><topic>Fundamental and applied biological sciences. 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Academic</collection><collection>Biotechnology Research Abstracts</collection><jtitle>Biotechnology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Park, Joo-Hyeon</au><au>Lee, Jin-Hyung</au><au>Kim, Chang-Jin</au><au>Lee, Jae-Chan</au><au>Cho, Moo Hwan</au><au>Lee, Jintae</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Extracellular protease in Actinomycetes culture supernatants inhibits and detaches Staphylococcus aureus biofilm formation</atitle><jtitle>Biotechnology letters</jtitle><addtitle>Biotechnol Lett</addtitle><date>2012-04-01</date><risdate>2012</risdate><volume>34</volume><issue>4</issue><spage>655</spage><epage>661</epage><pages>655-661</pages><issn>0141-5492</issn><eissn>1573-6776</eissn><coden>BILED3</coden><abstract>Bacterial biofilms are associated with chronic infections due to their resistance to antimicrobial agents. 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subjects	Actinobacteria - enzymology Actinomycetales Actinomycetes Anti-Bacterial Agents - metabolism antibiotic resistance Antimicrobial agents Bacteria Bacterial Adhesion - drug effects Bioengineering biofilm Biofilms Biofilms - drug effects Biofilms - growth & development Biological and medical sciences Biotechnology Cellular biology Culture Fundamental and applied biological sciences. Psychology Humans Inhibitors Medical equipment pathogens Peptide Hydrolases - metabolism Protease Proteases proteinases Staphylococcus aureus Staphylococcus aureus - drug effects Staphylococcus aureus - physiology Staphylococcus infections Strain
title	Extracellular protease in Actinomycetes culture supernatants inhibits and detaches Staphylococcus aureus biofilm formation
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