Determination of the ribosome-binding sequence and spacer length between binding site and initiation codon for efficient protein expression in Bifidobacterium longum 105-A

The frequency of the ribosome-binding site (RBS) and spacer length between binding site and initiation codon were analyzed using informatics, and compared the activities of various synthetic RBSs and spacers in Bifidobacterium longum 105-A. As the result, AAGGAG and a 5nt spacer length produced the...

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Bibliographic Details
Published in:Journal of bioscience and bioengineering 2012-04, Vol.113 (4), p.442-444
Main Authors: He, Jianlong, Sakaguchi, Kouta, Suzuki, Tohru
Format: Article
Language:English
Subjects:
Bacterial Proteins - biosynthesis
Bacterial Proteins - chemistry
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Bifidobacterium
Bifidobacterium - genetics
Bifidobacterium - metabolism
Bifidobacterium longum
binding sites
Binding Sites - genetics
Biological and medical sciences
Biotechnology
Codon, Initiator - chemistry
Codon, Initiator - genetics
Codon, Initiator - metabolism
Codons
DNA, Intergenic - chemistry
DNA, Intergenic - genetics
Fundamental and applied biological sciences. Psychology
Protein Biosynthesis - genetics
protein synthesis
RBS
Ribosomes - genetics
Ribosomes - metabolism
RNA, Messenger - chemistry
RNA, Messenger - genetics
RNA, Messenger - metabolism
Shine-Dalgarno sequence
start codon
Translation
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Summary:The frequency of the ribosome-binding site (RBS) and spacer length between binding site and initiation codon were analyzed using informatics, and compared the activities of various synthetic RBSs and spacers in Bifidobacterium longum 105-A. As the result, AAGGAG and a 5nt spacer length produced the most efficient protein expression.
ISSN:1389-1723
1347-4421
DOI:10.1016/j.jbiosc.2011.11.019