Siderophore production by actinomycetes isolates from two soil sites in Western Australia

The actinomycetes are metabolically flexible soil micro-organisms capable of producing a range of compounds of interest, including siderophores. Siderophore production by actinomycetes sampled from two distinct and separate geographical sites in Western Australia were investigated and found to be ge...

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Bibliographic Details
Published in:Biometals 2012-04, Vol.25 (2), p.285-296
Main Authors: Lee, Joanna, Postmaster, Armin, Soon, Hooi Peng, Keast, David, Carson, Kerry C.
Format: Article
Language:English
Subjects:
Actinobacteria - genetics
Actinobacteria - metabolism
Bacteria
Bacterial Proteins - genetics
Bacterial Proteins - isolation & purification
Bacterial Proteins - metabolism
Biochemistry
Biomedical and Life Sciences
Cell Biology
Chromatography, High Pressure Liquid
Enterobacteriaceae
Enterobactin - chemistry
Iron
Iron - pharmacology
Laterites
Life Sciences
Medicine/Public Health
Microbiology
Nocardia
Orchards
Pharmacology/Toxicology
Plant Physiology
Rhodococcus
Sandy loam
Siderophores - analysis
Siderophores - biosynthesis
Soil Microbiology
Soil microorganisms
Streptomyces
Western Australia
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Summary:The actinomycetes are metabolically flexible soil micro-organisms capable of producing a range of compounds of interest, including siderophores. Siderophore production by actinomycetes sampled from two distinct and separate geographical sites in Western Australia were investigated and found to be generally similar in the total percentage of siderophore producers found. The only notable difference was the proportion of isolates producing catechol siderophores with only 3% found in site 1 (from the north-west of Western Australia and reportedly containing 40% magnetite) and 17% in site 2 (a commercial stone fruit orchard in the hills east of Perth with a soil base ranging from sandy loam to laterite). Further detailed characterization of isolates of interest identified a Streptomyces that produced extracellularly excreted enterobactin, the characteristic Enterobacteriaceae siderophore, and also revealed some of the conditions required for enterobactin production. Carriage of the entF gene, which codes for the synthetase responsible for the final assembly of the tri-cyclic structure of enterobactin, was confirmed by PCR in this isolate. Another separate Streptomyces produced a compound that matched the UV/VIS spectra of heterobactin, a siderophore previously only described in Rhodococcus and Nocardia .
ISSN:0966-0844
1572-8773
DOI:10.1007/s10534-011-9503-9