Somaclonal variation in the progeny of transgenic barley

Somaclonal variation (SCV) in transgenic plants may slow the incorporation of introduced genes into commercially competitive cultivars. Somaclonal variation in transgenic barley (Hordeum vulgare L.) was assessed in one experiment by comparing the agronomic characteristics of 44 segregating transgeni...

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Bibliographic Details
Published in:Theoretical and applied genetics 1998-03, Vol.96 (3/4), p.421-425
Main Authors: Bregitzer, P, Halbert, S.E, Lemaux, P.G
Format: Article
Language:English
Subjects:
agronomic traits
bar gene
Barley
beta-glucuronidase
Biological and medical sciences
Classical genetics, quantitative genetics, hybrids
Comparative analysis
crop yield
Fundamental and applied biological sciences. Psychology
Genes
Genetic engineering
genetic transformation
Genetics of eukaryotes. Biological and molecular evolution
glufosinate
heading date
height
herbicide resistance
Hordeum vulgare
plant characteristics
Pteridophyta, spermatophyta
reporter genes
seed weight
somaclonal variation
tissue culture
Transgenic plants
uida gene
Vegetals
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Summary:Somaclonal variation (SCV) in transgenic plants may slow the incorporation of introduced genes into commercially competitive cultivars. Somaclonal variation in transgenic barley (Hordeum vulgare L.) was assessed in one experiment by comparing the agronomic characteristics of 44 segregating transgenic lines in the T2 generation to their non-transformed parent ('Golden Promise'). A second experiment examined the agronomic characteristics of seven transgenic-derived, null (non-transgenic) segregant lines in the T2 and T4 generations. Compared to their uncultured parent, Golden Promise, most of these lines were shorter, lower yielding, and had smaller seed, and the variability among individual plants was higher. The frequency and severity of the observed SCV was unexpectedly high, and the transformation procedure appeared to induce greater SCV than tissue culture in the absence of transformation. Attempts to understand the sources of SCV, and to modify transformation procedures to reduce the generation of SCV, should be made.
ISSN:0040-5752
1432-2242
DOI:10.1007/s001220050758