Effects of Azospirillum brasilense Sp7 Lectin and Phaseolus vulgarus Phytohemagglutinin on Cytokine Activity of Lymphocytes in Vitro

• Published in: Bulletin of experimental biology and medicine 2012, Vol.152 (3), p.333-335
• Main Authors: Ponomareva, E. G., Emelyanova, N. V., Nikitina, V. E.
• Format: Article
• Language: English
• Subjects: Agglutinins; alpha -Interferon; Azospirillum brasilense; Azospirillum brasilense - chemistry; Biomedical and Life Sciences; Biomedicine; Blood; Cell Biology; Centrifugation, Density Gradient; Cytokines - metabolism; Enzyme-Linked Immunosorbent Assay; gamma -Interferon; Humans; Immunology and Microbiology; Immunomodulation; Immunostimulation; Interferon; Interferon-alpha - metabolism; Interferon-gamma - metabolism; Interleukin 1; Interleukin 4; Interleukin 8; Interleukin-1alpha - metabolism; Interleukin-4 - metabolism; Interleukin-8 - metabolism; Internal Medicine; Laboratory Medicine; Lectins; Lectins - pharmacology; Lymphocytes; Lymphocytes - drug effects; Lymphocytes - metabolism; Pathology; Phaseolus; Phaseolus - chemistry; phytohemagglutinins; Phytohemagglutinins - pharmacology; Tumor necrosis factor; Tumor necrosis factor- alpha; Tumor Necrosis Factor-alpha - metabolism
• ISSN: 0007-4888; 1573-8221
• DOI: 10.1007/s10517-012-1521-2

We studied the effect of fucose-specific lectin from Azospirillum brasilense Sp7 bacteria and Phaseolus vulgarus phytohemagglutinin (PHA) on activity of IFN-γ, IFN-α, IL-1α, IL-4, IL-8, and TNF-α in human blood in vitro . During the experiment, IFN-γ production increased from 25 pg/ml in controls to 103 and 56 pg/ml under the infl uence of lectin and PHA, respectively. Agglutinins had similar effects on IFN-α production. Bacterial lectin increased IL-1α level from 2.65 ± 0.08 to 8.45 ± 0.41 pg/ml. PHA also increased IL-1α levels, but this effect was by 1.5–2 times less pronounced. Bacterial lectin and PHA increased production IL-4 by almost 2 times and production of IL-8 by 6 and 5 times, respectively, compared to the control. No differences were found in the effects of bacterial lectin and PHA on TNF-α synthesis. Experiments showed that immunostimulatory and immunomodulatory effects of bacterial lectin are more pronounced than those of PHA.