Genotype identification and assessment of genetic relationships in pearl millet [Pennisetum glaucum (L.) R. Br] using microsatellites and RAPDs

The potential of DNA markers such as microsatellites, minisatellites and RAPDs was investigated in pearl millet [Pennisetum glaucum (L.) R. Br] with respect to their abundance and variability. Southern analysis, using 22 different di-, tri-, tetra- and penta- oligonucleotide probes and five minisate...

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Bibliographic Details
Published in:Theoretical and applied genetics 1998-07, Vol.97 (1/2), p.154-162
Main Authors: Chowdari, K.V, Davierwala, A.P, Gupta, V.S, Ranjekar, P.K, Govila, O.P
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cenchrus americanus
Classical genetics, quantitative genetics, hybrids
cultivars
Deoxyribonucleic acid
DNA
Fundamental and applied biological sciences. Psychology
Genetic aspects
Genetic diversity
genetic markers
genetic polymorphism
genetic variation
Genetics of eukaryotes. Biological and molecular evolution
Genotype
Genotype & phenotype
identification
inbred lines
India
landraces
microsatellite repeats
Pearl millet
Pennisetum glaucum
Physiological aspects
Plant genetics
Pteridophyta, spermatophyta
Quantitative genetics
random amplified polymorphic DNA technique
taxonomy
Vegetals
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Summary:The potential of DNA markers such as microsatellites, minisatellites and RAPDs was investigated in pearl millet [Pennisetum glaucum (L.) R. Br] with respect to their abundance and variability. Southern analysis, using 22 different di-, tri-, tetra- and penta- oligonucleotide probes and five minisatellite probes, identified (GATA)4 as the most useful probe for the detection of multiple polymorphic fragments among pearl millet cultivars and landraces from India. The clustering patterns of pearl millet cultivars and landraces based on (GATA)4 and RAPD (randomly amplified polymorphic DNA) markers differed. The landraces, representing eight states in India, could not be grouped based on their geographical distribution with the DNA markers. RAPD analysis revealed a high degree of genetic diversity among the cultivars and landraces employed in this study. The probability of an identical match by chance for any two genotypes using (GATA)4 and RAPDs was 3.02 x 10(-20) for cultivars and 5.2 x 10(-9) for landraces. The microsatellite (GATA)4 and RAPDs provide useful tools for genotype identification and for the assessment of genetic relationships in pearl millet.
ISSN:0040-5752
1432-2242
DOI:10.1007/s001220050880