BVPaP-3, a T7-Like Lytic Phage of Pseudomonas aeruginosa: Its Isolation and Characterisation

The increasing emergence of antibiotic-resistant bacteria has produced a growing interest among scientists in bacteriophages as alternative antimicrobial agents. This article reports a lytic phage against an antibiotic-resistant strain of Pseudomonas aeruginosa. Phage BVPaP-3 is a member of the Podo...

Full description

Saved in:
Bibliographic Details
Published in:Current microbiology 2012-04, Vol.64 (4), p.305-311
Main Authors: Ahiwale, Sangeeta, Prakash, Divya, Gajbhiye, Milind, Jagdale, Smita, Patil, Nita, Kapadnis, Balu
Format: Article
Language:English
Subjects:
Adsorption
Antibiotic resistance
Antimicrobial agents
Bacteria
bacteriophages
Biomedical and Life Sciences
Biotechnology
Burst size
DNA
DNA, Viral - genetics
Drug resistance
Head
Host Specificity
Humans
Hydrogen-Ion Concentration
Life Sciences
Microbial Viability - drug effects
Microbial Viability - radiation effects
Microbiology
Molecular Weight
pH effects
Phage gh-1
Phages
Podoviridae
Podoviridae - genetics
Podoviridae - isolation & purification
Podoviridae - physiology
Podoviridae - ultrastructure
proteins
proteome
Proteome - analysis
Pseudomonas aeruginosa
Pseudomonas aeruginosa - virology
Pseudomonas Phages - genetics
Pseudomonas Phages - isolation & purification
Pseudomonas Phages - physiology
Pseudomonas Phages - ultrastructure
scientists
Tails
Temperature
Temperature effects
Viral Proteins - analysis
Viral Proteins - chemistry
Virion - ultrastructure
Viruses
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The increasing emergence of antibiotic-resistant bacteria has produced a growing interest among scientists in bacteriophages as alternative antimicrobial agents. This article reports a lytic phage against an antibiotic-resistant strain of Pseudomonas aeruginosa. Phage BVPaP-3 is a member of the Podoviridae family and morphologically similar to the T7-like phage gh-1. The phage has a hexagonal head of 58–59 nm in diameter and a short tail of 10 × 8 nm. It is stable at a wide range of pH (6–10) and temperatures (4–40°C). Its optimal growth temperature is 37°C and the adsorption rate constant is 1.19 × 10−9. Latent and eclipse periods are 20 and 15 min, respectively, and the burst size is 44 after 35 min at 37°C. The phage has a DNA size of 41.31 kb and a proteome of 11 proteins. The major protein is 33 kDa in size.
ISSN:0343-8651
1432-0991
DOI:10.1007/s00284-011-0071-6