Induction of atrazine resistance and somatic embryogenesis in Solanum melongena

The effects of atrazine on cotyledon cultures of Solanum melongena were investigated with a view to establishing a system for in vitro selection of resistant mutants. At herbicide levels producing little growth inhibition some chlorophyll loss occurred associated with the production of albino shoots...

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Bibliographic Details
Published in:Theoretical and applied genetics 1997-09, Vol.95 (4), p.702-705
Main Authors: ASHFAQ FAROOQUI, M, RAO, A. V, JAYASREE, T, SADANANDAM, A
Format: Article
Language:English
Subjects:
Atrazine
Biological and medical sciences
Classical genetics, quantitative genetics, hybrids
Eggplant
Embryonic development
Fundamental and applied biological sciences. Psychology
Genetic aspects
Genetics of eukaryotes. Biological and molecular evolution
Health aspects
Herbicide resistance
Herbicides
Physiological aspects
Plant genetics
Pteridophyta, spermatophyta
Solanum melongena
Sucrose
Vegetals
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Summary:The effects of atrazine on cotyledon cultures of Solanum melongena were investigated with a view to establishing a system for in vitro selection of resistant mutants. At herbicide levels producing little growth inhibition some chlorophyll loss occurred associated with the production of albino shoots. At 15 mg/l bleaching was more pronounced and was accompanied by the development of necrotic spots; however, efficient bleaching was associated with severe suppression of growth. Mutagenesis with EMS resulted in herbicide-resistant mutants based on the embryogenic ability of mutagenised explants placed on medium containing selective levels of sucrose (0.2%) and atrazine (15 mg/l). Different morphogenetic responses were observed when the levels of sucrose (0.2-5%) were altered. Somatic embryogenesis was observed at low sucrose concentrations (0.2-0.5%). Both embryogenesis and shoot regeneration occurred in 1% sucrose. Shoot regeneration was maximum in 2% sucrose and the regenerating ability decreased with a further increase in sucrose concentration (3-5%). However, lowering of sucrose concentration from 2% to 0.2% caused complete bleaching, permitting the selection of herbicide-resistant mutants.
ISSN:0040-5752
1432-2242
DOI:10.1007/s001220050615