Integrated map of AFLP, SSLP and RFLP markers using a recombinant inbred population of rice (Oryza sativa L.)

• Published in: Theoretical and applied genetics 1998-08, Vol.97 (3), p.370-380
• Main Authors: Cho, Y.G, McCouch, S.R, Kuiper, M, Kang, M.R, Pot, J, Groenen, J.T.M, Eun, M.Y
• Format: Article
• Language: English
• Subjects: amplified fragment length polymorphism; Biological and medical sciences; Biological markers; Chromosome mapping; Classical genetics, quantitative genetics, hybrids; Fundamental and applied biological sciences. Psychology; Genetic aspects; Genetic markers; Genetics; Genetics of eukaryotes. Biological and molecular evolution; Genomics; heterozygosity; Identification and classification; inbred lines; isozymes; loci; microsatellite repeats; Oryza sativa; Pteridophyta, spermatophyta; recombinant inbred lines; restriction fragment length polymorphism; Rice; segregation; sinple sequence length polymorphism; Vegetals
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/s001220050907

A molecular map of rice consisting of 231 amplified fragment length polymorphisms (AFLPs), 212 restriction fragment length polymorphisms (RFLPs), 86 simple-sequence length polymorphisms (SSLPs), five isozyme loci, and two morphological mutant loci [phenol staining of grain (Ph), semi-dwarf habit (sd-1)] has been constructed using an F11 recombinant inbred (RI) population. The mapping population consisted of 164 RI lines and was developed via single-seed descent from an intercross between the genetically divergent parents Milyang 23 (M) (tongil type) and Gihobyeo (G) (japonica type). A subset of previously mapped RFLP and SSLP markers were used to construct the map framework. The AFLP markers were derived from ten EcoRI(+2) and MseI(+3) primer combinations. All marker types were well distributed throughout the 12 chromosomes. The integrated map covered 1814 cM, with an average interval size of 3.4 cM. The MG map is a cornerstone of the Korean Rice Genome Research Program (KRGRP) and is being continuously refined through the addition of partially sequenced cDNA markers derived from an immature-seed cDNA library developed in Korea, and microsatellite markers developed at Cornell. The population is also being used for quantitative trait locus (QTL) analysis and as the basis for marker-assisted variety development.