Gel electrophoretic investigations of prolamins in eu- and alloplasmatic octoploid primary triticale forms

Unreduced prolamin fractions of eu- and alloplasmatic octoploid triticale forms were investigated by means of gel electrophoresis. The electrophoretic separation of the prolamin fractions was carried out by using one-dimensional, horizontal, native acidic polyacrylamide gel electrophoresis (APAGE) a...

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Bibliographic Details
Published in:Theoretical and applied genetics 1998, Vol.96 (1), p.46-51
Main Authors: Rozynek, B, Gunther, T, Hesemann, C.U
Format: Article
Language:English
Subjects:
acidic page
apage
Biological and medical sciences
Classical genetics, quantitative genetics, hybrids
Comparative analysis
comparisons
Fundamental and applied biological sciences. Psychology
Genetics of eukaryotes. Biological and molecular evolution
Genomes
gliadin
polyacrylamide gel electrophoresis
polyploidy
prolamins
protein composition
Pteridophyta, spermatophyta
Secale cereale
secalin
triticale
Triticum
Triticum aestivum
Vegetals
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Summary:Unreduced prolamin fractions of eu- and alloplasmatic octoploid triticale forms were investigated by means of gel electrophoresis. The electrophoretic separation of the prolamin fractions was carried out by using one-dimensional, horizontal, native acidic polyacrylamide gel electrophoresis (APAGE) and gradient gels. A comparison of the electropherogram patterns of triticale forms with those of the genome donors showed that most of the parent prolamins can be found again in the corresponding triticale. However, the bands of the triticale forms exhibited lower intensities than those of the genome donors. On average the gliadin bands of the triticale forms showed equal reductions in intensities, thereby preserving the relationships of the band intensities in most cases. On the other hand, secalin bands which came from the rye parent showed varying reductions in intensities. A comparative analysis of the band patterns revealed that differences could often be found between triticale forms with the same genomic constitution. Often it concerned differences in intensity. In some cases the bands showed changes in mobility or a splitting up into two sub-bands. Starting with the plant material presented here we developed a new nomenclature of the prolamin band patterns of the triticale forms.
ISSN:0040-5752
1432-2242
DOI:10.1007/s001220050707