Transgenic tobacco cultivars resistant to Pseudomonas syringae pv. tabaci

Six oriental cultivars of tobacco (Nicotiana tabacum L.) were evaluated for transformation and foreign gene expression. Leaf-disc explant tissue was transformed with Agrobacterium tumefaciens strain LBA4404 carrying the plasmid pARK21, which contains NPTII gene and ttr (tabtoxin resistance) gene con...

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Bibliographic Details
Published in:Theoretical and applied genetics 1998-10, Vol.97 (5/6), p.986-989
Main Authors: Batchvarova, R, Nikolaeva, V, Slavov, S, Bossolova, S, Valkov, V, Atanassova, S, Guelemerov, S, Atanassov, A, Anzai, H
Format: Article
Language:English
Subjects:
Agrobacterium tumefaciens
Bacteria
bacterial toxins
Biological and medical sciences
Classical genetics, quantitative genetics, hybrids
Cultivars
disease resistance
drug resistance
Fundamental and applied biological sciences. Psychology
Gene expression
gene transfer
genes
Genetic aspects
genetic resistance
genetic transformation
Genetics of eukaryotes. Biological and molecular evolution
Health aspects
inheritance (genetics)
kanamycin
kinases
leaves
neomycin phosphotransferase ii
Nicotiana tabacum
Physiological aspects
Plant genetics
Plant immunology
Pseudomonas syringae
Pseudomonas syringae pv. tabaci
Pteridophyta, spermatophyta
segregation
tabtoxin
Tobacco (Plant)
transgenic plants
ttr gene
Vegetals
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Summary:Six oriental cultivars of tobacco (Nicotiana tabacum L.) were evaluated for transformation and foreign gene expression. Leaf-disc explant tissue was transformed with Agrobacterium tumefaciens strain LBA4404 carrying the plasmid pARK21, which contains NPTII gene and ttr (tabtoxin resistance) gene conferring the resistance to Pseudomonas syringae pv. tabaci. The disease resistance of regenerated plants and segregation of this trait up to R7 progeny were investigated in a greenhouse and under field conditions. Our results indicated that the resistance to Pseudomonas syringae pv. tabaci introduced by transformation is heritable.
ISSN:0040-5752
1432-2242
DOI:10.1007/s001220050981