Molecular markers linked to white rust resistance in mustard Brassica juncea

• Published in: Theoretical and applied genetics 1998-10, Vol.97 (5/6), p.865-870
• Main Authors: Prabhu, K.V, Somers, D.J, Rakow, G, Gugel, R.K
• Format: Article
• Language: English
• Subjects: ac21 locus; Albugo candida; Biological and medical sciences; Brassica juncea; bulked segregant analysis; chromosome mapping; Classical genetics, quantitative genetics, hybrids; Cruciferae; cultivars; disease resistance; Fundamental and applied biological sciences. Psychology; Genetic aspects; Genetic markers; genetic techniques and protocols; Genetics; Genetics of eukaryotes. Biological and molecular evolution; Genomics; germplasm; Identification and classification; linkage (genetics); linkage groups; loci; Plant breeding; Plant genetics; Plant immunology; Pteridophyta, spermatophyta; random amplified polymorphic DNA technique; rust diseases; segregation; Vegetals
• ISSN: 0040-5752; 1432-2242
• DOI: 10.1007/s001220050966

White rust, caused by Albugo candida (Pers.) Kuntze, is an economically important disease of Brassica juncea (L.) Czern and Coss mustard, particularly in India. The most efficient and cost-effective way of protecting mustard plants from white rust disease is through genetic resistance. The objective of this study was to identify RAPD markers for white rust resistance in an F1-derived doubled-haploid (DH) population originating from a cross between white rust-susceptible and white rust-resistant breeding lines of B. juncea from the canola-quality B. juncea breeding project of the Agriculture and Agri-Food Canada-Saskatoon Research Centre. The DH population was used to screen for RAPD markers associated with white rust resistance/susceptibility using bulked segregant analysis. Two markers, WR2 and WR3, linked to white rust resistance, flanked the resistance locus Ac2(1) and were highly effective in identifying the presence or absence of the resistance gene in the DH population. These two markers were shown to be specific to the Russian source of white rust resistance utilized in this project. It is concluded that the availability of these RAPD markers will enhance the breeding for white rust resistance in B. juncea.