Alteration of beta-tubulin in Nicotiana plumbaginifolia confers resistance to amiprophos-methyl

A Nicotiana plumbaginifolia plant (apm5r) resistant to amiprophos-methyl (APM), a phosphoroamide herbicide, was isolated from protoplasts prepared from leaves of haploid plants. Genetic analysis revealed that the resistance is coded for by a dominant nuclear mutation and is associated with the incre...

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Bibliographic Details
Published in:Theoretical and applied genetics 1998-08, Vol.97 (3), p.464-472
Main Authors: Blume, Ya.B, Strashnyuk, N.M, Smertenko, A.P, Solodushko, V.G, Sidorov, V.A, Gleba, Yu.Yu
Format: Article
Language:English
Subjects:
amide herbicides
Biological and medical sciences
callus
Classical genetics, quantitative genetics, hybrids
Control
cortical microtubules
cross resistance
Fundamental and applied biological sciences. Psychology
gamma radiation
genes
Genetic aspects
Genetics of eukaryotes. Biological and molecular evolution
Herbicide resistance
in vitro selection
isoelectric point
leaves
microtubules
mutagenesis
Nicotiana
Nicotiana plumbaginifolia
Plant genetics
protoplasts
Pteridophyta, spermatophyta
stability
trifluralin
tubulin
Vegetals
Weeds
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Summary:A Nicotiana plumbaginifolia plant (apm5r) resistant to amiprophos-methyl (APM), a phosphoroamide herbicide, was isolated from protoplasts prepared from leaves of haploid plants. Genetic analysis revealed that the resistance is coded for by a dominant nuclear mutation and is associated with the increased stability of cortical microtubules. Two-dimensional polyacrylamide-gel electrophoresis, combined with immunoblotting using anti-tubulin monoclonal antibodies, showed that part of the beta-tubulin in the resistant plant possessed lower isoelectric points than the beta-tubulin of susceptible wild-type plants. These results provide evidence that the resistance to APM is associated with a mutation in a beta-tubulin gene. The APM-resistant line showed cross-resistance to trifluralin, a dinitroaniline herbicide, suggesting a common mechanism of resistance between these two classes of herbicides.
ISSN:0040-5752
1432-2242
DOI:10.1007/s001220050918