Characterisation of the radish introgression carrying the Rfo restorer gene for the Ogu-INRA cytoplasmic male sterility in rapeseed (Brassica napus L.)

Bulked segregant analysis and comparative mapping were applied to identify molecular markers inked to the Rfo restorer gene used for the Ogu-INRA cytoplasmic male-sterility system in rapeseed. These markers were then used to localise the radish introgression on the B. napus genetic map constructed f...

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Bibliographic Details
Published in:Theoretical and applied genetics 1998-07, Vol.97 (1/2), p.129-134
Main Authors: Delourme, R, Foisset, N, Horvais, R, Barret, P, Champagne, G, Cheung, W.Y, Landry, B.S, Renard, M
Format: Article
Language:English
Subjects:
Biological and medical sciences
Brassica napus
chemical constituents of plants
chromosome mapping
Classical genetics, quantitative genetics, hybrids
Cytogenetics
cytoplasmic male sterility
Daikon
Fundamental and applied biological sciences. Psychology
genes
Genetic aspects
Genetic markers
Genetics
Genetics of eukaryotes. Biological and molecular evolution
Genomics
glucosinolates
homologous recombination
intergeneric hybridization
introgression
linkage (genetics)
loci
Physiological aspects
Plant genetics
Pteridophyta, spermatophyta
quantitative traits
Radishes
random amplified polymorphic DNA technique
Rapeseed
Raphanus sativus
restorer genes
restriction fragment length polymorphism
seeds
segregation
Vegetals
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Summary:Bulked segregant analysis and comparative mapping were applied to identify molecular markers inked to the Rfo restorer gene used for the Ogu-INRA cytoplasmic male-sterility system in rapeseed. These markers were then used to localise the radish introgression on the B. napus genetic map constructed from the cross 'Darmor.bzh' x 'Yudal'. The introgression mapped on the DY15 linkage group. From the comparison of this latter group to the linkage group constructed on a F2 progeny segregating for the radish introgression, it was concluded that the introgression had occurred through homoeologous recombination, that it was not distal and that it had replaced a B. napus region of around 50 cM. A QTL involved in aliphatic seed glucosinolate content was located on the DY15 linkage group at a position corresponding to one end of the introgression. The DNA markers identified in this study are being used in map-based cloning of the Rfo gene and in marker-assisted selection.
ISSN:0040-5752
1432-2242
DOI:10.1007/s001220050876