Bifidobacterium Longum Modified Recombinant HU Protein as a Vector for Nonviral Delivery of DNA to HEK293 Human Cell Culture

Creation of effective nontoxic highly specific systems for nonviral transportation of DNA is one of the priority problems in the development of genotherapeutic methods. Chimerical recombinant proteins consisting of Antp and Tat protein cell-penetrating domains and Bifidobacterium longum DNA-binding...

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Bibliographic Details
Published in:Bulletin of experimental biology and medicine 2011-10, Vol.151 (6), p.717-721
Main Authors: Khokhlova, E. V., Efimov, B. A., Kafarskaia, L. I., Pavlov, K. A., Shkoporov, A. N.
Format: Article
Language:English
Subjects:
Bacterial Proteins - genetics
Bacterial Proteins - metabolism
Bifidobacterium - genetics
Bifidobacterium longum
Biomedical and Life Sciences
Biomedicine
Cell Biology
Cell culture
Cells
DNA
Electrophoresis, Polyacrylamide Gel
Gene therapy
Genetic Vectors - genetics
Genetics
Green fluorescent protein
HEK293 Cells
HU protein
Humans
Internal Medicine
Laboratory Medicine
Pathology
Peptides
Plasmids
Polymerase Chain Reaction
Protein binding
Recombinant proteins
Recombinant Proteins - genetics
Recombinant Proteins - metabolism
Tat protein
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Summary:Creation of effective nontoxic highly specific systems for nonviral transportation of DNA is one of the priority problems in the development of genotherapeutic methods. Chimerical recombinant proteins consisting of Antp and Tat protein cell-penetrating domains and Bifidobacterium longum DNA-binding histone-like protein HU were obtained. The resultant recombinant proteins bind to plasmid DNA in vitro and provide intracellular delivery and expression of the reporter genetic construction with GFP gene in cultured HEK293 human cells.
ISSN:0007-4888
1573-8221
DOI:10.1007/s10517-011-1424-7