Extracting genomic DNA of foodstuff by polyamidoamine (PAMAM)–magnetite nanoparticles

• Published in: Talanta (Oxford) 2012-05, Vol.93, p.166-171
• Main Authors: Qie, Fengxiang, Zhang, Guoxin, Hou, Jianxuan, Sun, Xiaoming, Luo, Shi-zhong, Tan, Tianwei
• Format: Article
• Language: English
• Subjects: Analytical chemistry; Chemical Fractionation - methods; Chemistry; Consumers; Dendrimers - chemistry; DNA - isolation & purification; Electrophoresis; Exact sciences and technology; Food, Genetically Modified; Foodstuff; Genome - genetics; Genomic DNA; Magnetite nanoparticles; Magnetite Nanoparticles - chemistry; PAMAM; Soy food; Static Electricity; Surface Properties
• ISSN: 0039-9140; 1873-3573
• DOI: 10.1016/j.talanta.2012.02.007

► The reliable identification of GM/non-GM food is important. ► The research develops a novel “single-pot” preparation of PMNPs. ► PMNPs successfully isolate genomic DNA from different sample foods. ► Screening GM and non-GM food is achieved. Although genetically modified (GM) food is becoming increasingly available, consumers are showing a growing awareness about the need to identify GM and non-GM foodstuff: the reliable identification of GM/non-GM food is therefore an important tool in the social, health and safety debates. The present research responds to this need (i) through developing a novel “single-pot” preparation of PAMAM magnetite nanoparticles (PMNPs) and by fully defining their specific characteristics; (ii) by demonstrating the capability of the PMNPs to isolate genomic DNA from different sample foods; and (iii) by experimentally demonstrating the identification of the isolated DNA by gel-electrophoresis, thus being capable of screening GM and non-GM food.