Surface plasmon resonance shows a gender difference in circulating annexin A5 in human

• Published in: Talanta (Oxford) 2012-05, Vol.93, p.219-223
• Main Authors: Trouvé, Pascal, Kerbiriou, Mathieu, le Hir, Sophie, Benz, Nathalie, Férec, Claude
• Format: Article
• Language: English
• Subjects: Analytical chemistry; Annexin A5; Annexin A5 - blood; Annexin A5 - immunology; Antibodies, Immobilized - immunology; Blood; Calibration; Chemistry; Circulating; Diseases; Emergency Medical Services; Exact sciences and technology; Female; Human; Human normal serum; Humans; Male; Males; Myocardial infarction; Plasmons; Reproducibility of Results; Serums; Sex Characteristics; Surface plasmon resonance; Surface Plasmon Resonance - methods
• ISSN: 0039-9140; 1873-3573
• DOI: 10.1016/j.talanta.2012.02.017

► Circulation annexin A5 may be measured in human serum using SPR. ► The measured levels of circulating annexin A5 by SPR are in the range of previous measurements made by ELISA. ► The level of circulating annexin A5 is higher in male than in female. The level of circulating anxA5 is correlated to various diseases such as acute myocardial infarction, trauma, thrombosis, inflammation and in some cancers. Our aim was to assess whether a direct approach using surface plasmon resonance (SPR) could be easily used to provide a rapid and cheap alternative to detect anxA5 in blood samples in human. Our results indicate that SPR permits to detect and quantify circulating anxA5 in serum with a minimum time of manipulation. Furthermore, we report here, for the first time, that the level of circulating anxA5 is significantly higher in male than in female (5.43 (±0.02) and 4.41 (±0.2)ng/ml, respectively). In conclusion, we found that SPR can be used to rapidly quantify anxA5 in patients and that a gender difference has to be taken into account to explain gender differences in the prevalence of some diseases.