Fungi associated with the southern Eurasian orchid Spiranthes spiralis (L.) Chevall

• Published in: Fungal biology 2012-04, Vol.116 (4), p.543-549
• Main Authors: Tondello, Alessandra, Vendramin, Elena, Villani, Mariacristina, Baldan, Barbara, Squartini, Andrea
• Format: Article
• Language: English
• Subjects: Alternaria; Ascomycota; Basidiomycota; Biodiversity; Bionectria ochroleuca; Davidiella; DNA, Fungal - chemistry; DNA, Fungal - genetics; DNA, Ribosomal Spacer - chemistry; DNA, Ribosomal Spacer - genetics; Endangered taxa; Endophytes; Endophytes - classification; Endophytes - cytology; Endophytes - genetics; Endophytes - isolation & purification; Endophytic fungi; Euganean Hills; Fungi; Fungi - classification; Fungi - cytology; Fungi - genetics; Fungi - isolation & purification; Fusarium oxysporum; Italy; Leptosphaeria; Malassezia; Molecular Sequence Data; Mycorrhizal symbionts; Orchidaceae; Orchidaceae - microbiology; Phylogeny; Plant Roots - microbiology; Rhizoctonia; Roots; Sequence Analysis, DNA; Spiranthes; Spiranthes spiralis
• ISSN: 1878-6146; 1878-6162
• DOI: 10.1016/j.funbio.2012.02.004

The hitherto unknown relationships between the European orchid Spiranthes spiralis (L.) Chevall and its internally associated fungi were explored by a combined approach involving microscopy-based investigations at a morpho-histological level as well as by molecular analyses of the identity of the eukaryotic endophytes present in the root tissue of the plant. We found that this orchid which is currently reported to have a vulnerable status in northern Italy, can host and interact with at least nine types of fungi. Some of these fungi show similarity to mycorrhizal genera found in orchids such as the Ceratobasidium-Rhizoctonia group. Other fungi found are from the genera Davidiella (Ascomycota), Leptosphaeria (Ascomycota), Alternaria (Ascomycota), and Malassezia (Basidiomycota), some of which until have not previously been reported to have an endophytic relationship with plants. The repeated occurrence of often pathogenic fungi such as Fusarium oxysporum, Bionectria ochroleuca, and Alternaria sp., within healthy specimens of this orchid suggests a tempered interaction with species that are sometimes deleterious to non-orchid plants. The fact is reminiscent of the symbiotic compromise established by orchids with fungi of the rhizoctonia group. ► The orchid Spiranthes spiralis can host at least nine types of endophytic fungi. ► These include the genera Ceratobasidium Davidiella, Leptosphaeria, Alternaria, Malassezia, Fusarium. ► Fungi that are pathogens elsewhere appear to dwell asymptomatically in S. spiralis.