A multifunctional teal-fluorescent Rosa26 reporter mouse line for Cre- and Flp-mediated recombination

• Published in: Neuroscience research 2012-05, Vol.73 (1), p.85-91
• Main Authors: Imayoshi, Itaru, Hirano, Kyoko, Sakamoto, Masayuki, Miyoshi, Goichi, Imura, Tetsuya, Kitano, Satsuki, Miyachi, Hitoshi, Kageyama, Ryoichiro
• Format: Article
• Language: English
• Subjects: Animals; Cre; DNA Nucleotidyltransferases - physiology; Flp; Gene Knock-In Techniques; Genes, Reporter - physiology; Green Fluorescent Proteins - physiology; Integrases - physiology; Knock-in mice; Mice; Mice, Inbred C57BL; Mice, Transgenic; mTFP1; Nervous system; Proteins - physiology; Recombination; Recombination, Genetic - physiology; RNA, Untranslated; Rosa26
• ISSN: 0168-0102; 1872-8111
• DOI: 10.1016/j.neures.2012.02.003

► We generated a new Cre/Flp fluorescent reporter mouse strain (R26-CAG-LF-mTFP1). ► To meet the need for single recombinase-mediated reporter expression, we generated derivatives of R26-CAG-LF-mTFP1. ► We evaluated these reporter mouse strains in the central nervous system. Reporters of Cre and/or Flp activity are important for defining the spatial and temporal extent of Cre/Flp-mediated recombination. Here, we describe R26-CAG-LF-mTFP1, a multifunctional fluorescent reporter mouse that strongly expresses mTFP1 (bright teal fluorescent protein) after Cre- and Flp-mediated recombination. To meet the need for single recombinase-mediated reporter expression, we generated derivatives of R26-CAG-LF-mTFP1. The germline excision of the Frt-flanked stop cassette in R26-CAG-LF-mTFP1 generated a Cre-dependent reporter (R26-CAG-LoxP-mTFP1). Similarly, R26-CAG-FRT-mTFP1, in which the loxP-flanked stop cassette was excised in the germline, requires only Flp to activate mTFP1 expression.