Allergen Challenge Differentially Affects the Number of Circulating Monocyte Subsets

Peripheral blood monocyte (PBM) subsets play different roles in inflammatory response and tissue remodelling. The aim of this study was to investigate how allergen challenge affects the number of circulating PBMs in Dermatophagoides pteronyssinus (Dp) allergic patients (Dp‐APs). Among 34 Dp‐APs chal...

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Bibliographic Details
Published in:Scandinavian journal of immunology 2012-05, Vol.75 (5), p.531-539
Main Authors: Kowal, K., Moniuszko, M., Dabrowska, M., Bodzenta-Lukaszyk, A.
Format: Article
Language:English
Subjects:
Adolescent
Adult
Allergens
Animals
Antigens, Dermatophagoides - immunology
Bronchial Provocation Tests
Bronchoconstriction
CCL17 protein
CD14 antigen
CD16 antigen
Chemokine CCL17 - blood
Chemokine CCL2 - blood
Chemokine CX3CL1 - blood
Dermatophagoides pteronyssinus
Dermatophagoides pteronyssinus - immunology
Enzyme-linked immunosorbent assay
Female
Flow cytometry
Histamine - administration & dosage
Histamine - immunology
Humans
Hypersensitivity - immunology
Inflammation
Lipopolysaccharide Receptors - blood
Male
Monocyte chemoattractant protein 1
Monocytes
Monocytes - immunology
Monocytes - metabolism
Peripheral blood
Receptors, CCR4 - blood
Receptors, IgG - blood
Respiratory tract
Young Adult
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Summary:Peripheral blood monocyte (PBM) subsets play different roles in inflammatory response and tissue remodelling. The aim of this study was to investigate how allergen challenge affects the number of circulating PBMs in Dermatophagoides pteronyssinus (Dp) allergic patients (Dp‐APs). Among 34 Dp‐APs challenged, in 22 patients significant bronchoconstriction was demonstrated [responders (Rs)], while in 12, only upper respiratory symptoms were seen [non‐responders (NRs)]. Twelve healthy, non‐atopic subjects were used as controls (HCs). Expression of CD14, CD16 and CCR4 was evaluated by flow cytometry on the whole‐blood samples before (T0), 6 h (T6) and 24 h (T24) after the challenge. Plasma concentrations of CCL2, CX3CL1 and CCL17 were evaluated using ELISA. At T0, the mean percentage of CD14++ CD16+ PBMs in Rs (35.4%; 95%CI 26.9–43.9%) was significantly greater than in HCs (14.6%; 95%CI 7.3–21.8%; P = 0.006) and in NRs (17.5%; 95%CI 9.6–25.4%; P = 0.001). The baseline number of CD14++ CD16+ PBMs correlated with airway hyper responsiveness (AHR) (r = −0.507; 95%CI −0.834 to −0.432, P 
ISSN:0300-9475
1365-3083
DOI:10.1111/j.1365-3083.2012.02685.x