Apolipoprotein E influences melatonin biosynthesis by regulating NAT and MAOA expression in C6 cells

:  Previous studies have demonstrated that apolipoprotein E (ApoE) genotype and melatonin are closely associated with Alzheimer’s disease (AD). However, the relationship between ApoE genotype and melatonin remains unclear. Recently, we reported that cultured rat cortical astrocytes and glioma C6 cel...

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Bibliographic Details
Published in:Journal of pineal research 2012-05, Vol.52 (4), p.397-402
Main Authors: Liu, Ya-Jing, Meng, Fan-Tao, Wang, Li-Li, Zhang, Li-Feng, Cheng, Xin-Ping, Zhou, Jiang-Ning
Format: Article
Language:English
Subjects:
Animals
apolipoprotein E
Apolipoproteins E - genetics
Apolipoproteins E - metabolism
Arylamine N-Acetyltransferase - genetics
Arylamine N-Acetyltransferase - metabolism
Astrocytes - metabolism
Blotting, Western
Cell Line, Tumor
Cells, Cultured
Flow Cytometry
Genotype
Humans
Isoenzymes - genetics
Isoenzymes - metabolism
melatonin
Melatonin - biosynthesis
Melatonin - genetics
Melatonin - metabolism
Monoamine Oxidase - genetics
Monoamine Oxidase - metabolism
monoamine oxidase A
monoamine oxidase B
N-acetyltransferase
Rats
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Summary::  Previous studies have demonstrated that apolipoprotein E (ApoE) genotype and melatonin are closely associated with Alzheimer’s disease (AD). However, the relationship between ApoE genotype and melatonin remains unclear. Recently, we reported that cultured rat cortical astrocytes and glioma C6 cells synthesize melatonin. In the current study, we investigated the effect of ApoE genotype on melatonin biosynthesis. C6 cells with stable expression of ApoE isoforms (ApoE 2, 3 and 4) were established. A higher level of melatonin was demonstrated in cultured ApoE4‐C6 cells than that in ApoE3‐C6 cells. In addition, we found that N‐acetyltransferase (NAT) protein level was up‐regulated in ApoE4‐C6 cells compared with ApoE3‐C6 cells. Further study suggested that mRNA expression of monoamine oxidase A (MAOA) and monoamine oxidase B (MAOB) decreased in ApoE4‐C6 cells. In conclusion, the increased melatonin level in ApoE4‐C6 cells results from up‐regulation of NAT expression, a key enzyme for melatonin synthesis, and down‐regulation of MAOA and MAOB expression, the metabolic enzyme for its precursor serotonin.
ISSN:0742-3098
1600-079X
DOI:10.1111/j.1600-079X.2011.00954.x