Site-Selective Affinity Labelling of Maltose Binding Protein in Bacterial Cells

Labelling simplified: In two steps, a thioester carbohydrate conjugate can be synthesised that site‐specifically labels maltose binding protein (shown in grey). Specific labelling is observed in crude cell extracts and within live E. coli cells.

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Bibliographic Details
Published in:Chembiochem : a European journal of chemical biology 2012-04, Vol.13 (6), p.783-787
Main Authors: Rullo, Anthony, Beharry, Andrew A., Gómez-Biagi, Rodolfo F., Zhao, Xiong, Nitz, Mark
Format: Article
Language:English
Subjects:
Affinity Labels - chemistry
Bacterial Proteins - chemistry
Bacterial Proteins - metabolism
bioorganic chemistry
glycoconjugates
Maltose - metabolism
Maltose-Binding Proteins - chemistry
Maltose-Binding Proteins - metabolism
Models, Molecular
protein conjugation
protein modifications
Spectrophotometry, Ultraviolet
Substrate Specificity
thioesters
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Description
Summary:Labelling simplified: In two steps, a thioester carbohydrate conjugate can be synthesised that site‐specifically labels maltose binding protein (shown in grey). Specific labelling is observed in crude cell extracts and within live E. coli cells.
ISSN:1439-4227
1439-7633
DOI:10.1002/cbic.201200046