Ultrahigh-Efficiency Protein Separations with Submicrometer Silica Using Slip Flow

[...]slip flow always occurs in reversedphase LC, but it is not distinguishable from Hagen-Poiseuille flow because the dimensions of chromatographic materials are relatively large. Experimentally, the parabolic flow profile cannot be directly viewed on the nanoscale, but it can be inferred because o...

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Bibliographic Details
Published in:LC GC North America 2012-10, Vol.30 (10), p.890
Main Authors: ROGERS, Benjamin J, WEI, Bingchuan, WIRTH, Mary J, MAJORS, Ronald E
Format: Magazinearticle
Language:English
Subjects:
Analytical chemistry
Analytical, structural and metabolic biochemistry
Atoms & subatomic particles
Biological and medical sciences
Capillary electrophoresis
Chromatography
Efficiency
Fluid dynamics
Fundamental and applied biological sciences. Psychology
General aspects, investigation methods
Hydrocarbons
Monoclonal antibodies
Proteins
Velocity
Viscosity
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Summary:[...]slip flow always occurs in reversedphase LC, but it is not distinguishable from Hagen-Poiseuille flow because the dimensions of chromatographic materials are relatively large. Experimentally, the parabolic flow profile cannot be directly viewed on the nanoscale, but it can be inferred because one can measure a companion to the narrower flow profile: a faster flow rate. Because slip flow gives a nonzero velocity at the wall, the volume flow rate, Q, is enhanced relative to that for Hagen-Poiseuille flow, Qj^p- In equation 1, for a capillary of radius r, the term L is the slip length, which is defined as the ratio of the velocity to shear rate, each at the wall. ..
ISSN:1527-5949
1939-1889