Back to the Basics: Considering Carryover, Column Back Pressure, and Wavelength When Developing Chromatographic Methods

High-performance liquid chromatography (HPLC) is a sophisticated technique. Chromatographers must not forget the basics of method development to reap the full benefits in practice. Obvious parameters to optimize include the choice of chromatographic column, mobile phase, injection volume, flow rate,...

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Bibliographic Details
Published in:LC GC North America 2013-04, Vol.31 (S4a), p.50
Main Authors: Usher, Karyn M, Hansen, Steven W, McNally, Mary Ellen P
Format: Article
Language:English
Subjects:
Analytical chemistry
Calibration
Chromatography
Experiments
Ions
Methods
Sensors
Solvents
Standard deviation
Technological change
Online Access:Get full text
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Summary:High-performance liquid chromatography (HPLC) is a sophisticated technique. Chromatographers must not forget the basics of method development to reap the full benefits in practice. Obvious parameters to optimize include the choice of chromatographic column, mobile phase, injection volume, flow rate, and detector settings. However, it is also beneficial to know how to determine carryover, the properties of the needle wash solvent used, and the importance of monitoring instrument back pressure. This article will discuss issues related to carryover, instrument back pressure, and the choice of wavelength. [PUBLICATION ABSTRACT]
ISSN:1527-5949
1939-1889