Food Grade Dye for Assessment of Biofilm Removal from Stainless Steel by Cleaning and Sanitizing Agents

The objective of this study was to evaluate a food grade dye, erythrosin B, for use in the development of a quantitative color difference methodology to measure the efficacy of cleaner/sanitizer solutions in removing biofilm components from stainless steel surfaces. Biofilms of Listeria innocua and...

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Bibliographic Details
Published in:Food Protection Trends 2019-11, Vol.39 (6), p.442-448
Main Authors: Gamble, Gary R, Lawrence, Kurt C, Park, Bosoon, Yoon, Seung-Chul, Heitschmidt, Gerald W
Format: Magazinearticle
Language:English
Subjects:
Bacteria
Biofilms
Cleaning
Cleaning agents
Color
Dyeing
Dyes
Evaluation
Food
Food contamination & poisoning
Food dyes
Food processing
Food safety
Hazard analysis critical control point
Hazard assessment
Inspection
Listeria
Listeria innocua
Methods
Poultry
Pseudomonas putida
Quality
Sanitation
Sanitizers
Stainless steel
Stainless steels
Visual perception
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Summary:The objective of this study was to evaluate a food grade dye, erythrosin B, for use in the development of a quantitative color difference methodology to measure the efficacy of cleaner/sanitizer solutions in removing biofilm components from stainless steel surfaces. Biofilms of Listeria innocua and Pseudomonas putida were grown on stainless-steel coupons, subjected to various cleaner/sanitizer treatments, and then stained with erythrosin B. Resultant coupons were photographed and mean L*a*b* color differences between background and dyed area evaluated. Color differences conformed to a scale correlated with human visual perception. Results indicated that the method provides sensitivity for visual appraisal of treatment-response as well as species-response relationships. The method shows potential as an enhancement for quantitative visual assessment of cleaning/sanitizing treatments of biofilms in a laboratory setting, and supplemental research is warranted to assess its efficacy for GRAS inspection of food processing environments as part of the Hazard Analysis Critical Control Point program.
ISSN:1541-9576