Separation and Sizing of a Virus Mixture Using Asymmetrical Flow Field-Flow Fractionation Coupled to Multi-Angle Light Scattering

Biogen and Postnova present data on the analysis of adeno-associated viruses (AAV) using Asymmetrical Flow Field-Flow Fractionation coupled to Multi-Angle Light Scattering detection (AF4-MALS). Due to their relatively large size, often over 50 nm in diameter, virus aggregates are challenging to sepa...

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Bibliographic Details
Published in:LC GC North America 2020-09, Vol.38 (9), p.534-535
Main Authors: Bou-Assaf, George, Blum, Andy, Matalka, Omar, Frenkel, Ruth, Reed, Robert, Tadjiki, Soheyl
Format: Magazinearticle
Language:English
Subjects:
Aggregates
Asymmetry
Chromatography
Column chromatography
Diameters
Flow
Fractionation
Gene therapy
Light scattering
Mixtures
Separation
Size exclusion chromatography
Viruses
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Summary:Biogen and Postnova present data on the analysis of adeno-associated viruses (AAV) using Asymmetrical Flow Field-Flow Fractionation coupled to Multi-Angle Light Scattering detection (AF4-MALS). Due to their relatively large size, often over 50 nm in diameter, virus aggregates are challenging to separate and characterize by column-based chromatography techniques such as size-exclusion chromatography (SEC). In this application note, we present data on separation of a virus mixture using Asymmetrical Flow Field-Flow Fractionation (AF4) and measurement of their radius of gyration (R ).
ISSN:1527-5949
1939-1889