Basic Principles for Purification Using Supercritical Fluid Chromatography

In particular, the patented modifier stream injection mode* is employed for all Waters preparative SFC systems, minimizing the solvent effect and therefore improving throughput and efficiency. * US Patent #6,5/6,125 CONCLUSIONS * The basic principles of liquid chromatography can be applied to superc...

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Bibliographic Details
Published in:LC GC North America 2010-11, Vol.28 (11), p.S15
Main Authors: Jablonski, Jo-Ann M, Hudalla, Christopher J, Fountain, Kenneth J, Collier, Steven M, Morrison, Damian
Format: Article
Language:English
Subjects:
Analytical chemistry
Chromatography
Efficiency
Nonsteroidal anti-inflammatory drugs
Particle size
Solvents
Online Access:Get full text
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Summary:In particular, the patented modifier stream injection mode* is employed for all Waters preparative SFC systems, minimizing the solvent effect and therefore improving throughput and efficiency. * US Patent #6,5/6,125 CONCLUSIONS * The basic principles of liquid chromatography can be applied to supercritical fluid chromatography (SFC). * Supercritical fluid chromatography can be run at increased flow rates making isolations faster and increasing throughput. * Samples with limited solubility can be dissolved in dimethyl sulfoxide and purified using SFC. Screening different columns with fast grathents leads to a decision about which preparative column to use for isolating the compounds in the sample. * The ability to scale between analytical and preparative OBD columns leads to identical separations at both the small and large scales, provided that the injection volume, slope, and grathent time are scaled correctly and the chemistry and particle size remain constant.
ISSN:1527-5949
1939-1889