Cloning and expression of a cDNA encoding the laccase from Schizophyllum commune

We cloned and analyzed the nucleotide sequence of a cDNA that encodes polyphenol oxidase (laccase) from the white-rot basidiomycete Schizophyllum commune. The nucleotide sequence of the full-length CDNA contained a 1554-base open reading frame that encoded a polypeptide of 518 amino acid residues, i...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 1999-01, Vol.63 (1), p.58-64
Main Authors: Hatamoto, O. (Noda Inst. for Scientific Research, Chiba (Japan)), Sekine, H, Nakano, E, Abe, K
Format: Article
Language:English
Subjects:
Amino Acid Sequence
ASPERGILLUS
Aspergillus - enzymology
Aspergillus - genetics
Base Sequence
Biological and medical sciences
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
CLONACION MOLECULAR
CLONAGE MOLECULAIRE
Cloning, Molecular
Conserved Sequence
DNA Primers - genetics
DNA, Complementary - genetics
DNA, Fungal - genetics
EXPRESION GENICA
EXPRESSION DES GENES
Fundamental and applied biological sciences. Psychology
GENE EXPRESSION
Genes, Fungal
Genetic engineering
Genetic technics
Genetics
LACASA
LACCASE
Methods. Procedures. Technologies
Mission oriented research
Modification of gene expression level
MOLECULAR CLONING
Molecular Sequence Data
NUCLEOTIDE SEQUENCE
Oxidoreductases - genetics
Phylogeny
polyphenol oxidase
Schizophyllum - enzymology
Schizophyllum - genetics
SCHIZOPHYLLUM COMMUNE
SECUENCIA NUCLEOTIDICA
SEQUENCE NUCLEOTIDIQUE
tannase
Transformation, Genetic
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Summary:We cloned and analyzed the nucleotide sequence of a cDNA that encodes polyphenol oxidase (laccase) from the white-rot basidiomycete Schizophyllum commune. The nucleotide sequence of the full-length CDNA contained a 1554-base open reading frame that encoded a polypeptide of 518 amino acid residues, including a putative signal peptide of 16 residues. It contained four highly similar regions that are conserved in the deduced amino acid sequences of other laccases, including the region thought to be involved in copper binding. Aspergillus sojae strain 1860 (which has low protease levels) was transformed with the plasmid lacAL/pTPT, which contained the laccase gene under the control of the tannase promoter from Aspergillus oryzae. Laccase was secreted into the medium when transformants Al and A2 were cultured in tannic acid-containing medium
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.63.58