Cloning and Sequencing of β-Mannosidase Gene from Aspergillus aculeatus No. F-50

The manB gene, coding for a unique β-mannosidase (MANB) of Aspergillus aculeatus, was cloned from genomic and cDNA libraries, and sequenced. The gene consists of 2,811 bp encoding a polypeptide of 937 amino acid residues with a molecular mass of 104,214 Da. The A. aculeatus MANB shared amino acid se...

Full description

Saved in:
Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 1999-01, Vol.63 (1), p.206-209
Main Authors: TAKADA, Goro, KAWAGUCHI, Takashi, KAGA, Tomoko, SUMITANI, Jun-ichi, ARAI, Motoo
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Aspergillus aculeatus
Base Sequence
beta-Mannosidase
Biological and medical sciences
Biology of microorganisms of confirmed or potential industrial interest
Biotechnology
Caenorhabditis elegans - enzymology
Caenorhabditis elegans - genetics
Cattle
Cloning, Molecular
DNA, Complementary - genetics
DNA, Fungal - genetics
Fundamental and applied biological sciences. Psychology
Genes, Fungal
Genetics
Goats
Humans
manB gene
Mannosidases - chemistry
Mannosidases - genetics
Mission oriented research
Molecular Sequence Data
Molecular Weight
Sequence Homology, Amino Acid
β-galactosidase
β-guclonidase
β-mannosidase
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The manB gene, coding for a unique β-mannosidase (MANB) of Aspergillus aculeatus, was cloned from genomic and cDNA libraries, and sequenced. The gene consists of 2,811 bp encoding a polypeptide of 937 amino acid residues with a molecular mass of 104,214 Da. The A. aculeatus MANB shared amino acid sequence identity with MANB of human (24%), goat (24%), bovine (24%), and Caenorhabditis elegans (22%). When the A. aculeatus MANB was compared with other related enzymes, a Glu residue corresponding to the active site identified by the Escherichia coli β-galactosidase and the human β-gluclonidase was conserved. This is the first fungal gene that encodes MANB.
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.63.206