High-performance liquid chromatographic validated assay of doxorubicin in rat plasma and tissues

A specific and selective high-performance liquid chromatography (HPLC) technique that requires few manipulations, and is readily adaptable to analysis for a large series of samples, has been developed for the determination of the concentration of the anticancer drug doxorubicin (DXR) in rat serum an...

Full description

Saved in:
Bibliographic Details
Published in:Journal of chromatography. B, Biomedical sciences and applications Biomedical sciences and applications, 1999-01, Vol.721 (2), p.271-278
Main Authors: Álvarez-Cedrón, Loreto, Sayalero, M a Luisa, Lanao, José M.
Format: Article
Language:English
Subjects:
Analysis
Animals
Antibiotics, Antineoplastic - analysis
Antibiotics, Antineoplastic - pharmacokinetics
Biological and medical sciences
Chromatography, High Pressure Liquid
Doxorubicin
Doxorubicin - analysis
Doxorubicin - pharmacokinetics
General pharmacology
Humans
Indicators and Reagents
Medical sciences
Pharmacology. Drug treatments
Rats
Rats, Wistar
Reference Standards
Reproducibility of Results
Solutions
Spectrometry, Fluorescence
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A specific and selective high-performance liquid chromatography (HPLC) technique that requires few manipulations, and is readily adaptable to analysis for a large series of samples, has been developed for the determination of the concentration of the anticancer drug doxorubicin (DXR) in rat serum and tissues. The biological samples were efficiently deproteinised and resolved from a reversed-phase nucleosil C 18 column with fluorescence detection. The validation study of the proposed method was successfully carried out in an assay range of between 5 and 5000 ng/ml and was subsequently implemented in a pharmacokinetic study of DXR in Wistar rats that were treated by intravenous administration of the drug.
ISSN:0378-4347
1387-2273
DOI:10.1016/S0378-4347(98)00475-7