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Inhibitory effects of cadmium on carbonic anhydrase activity and ionic regulation of the estuarine crab Chasmagnathus granulata (Decapoda, Grapsidae)
This work was aimed at evaluating the gill carbonic anhydrase (CA) activity of the estuarine crab Chasmagnathus granulata exposed in vivo to cadmium, at different salinities. The in vivo effect of the specific inhibitor acetazolamide (AZ) was also assayed. Besides, the inhibition of CA activity by d...
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Published in: | Comparative biochemistry and physiology. C, Comparative pharmacology and toxicology Comparative pharmacology and toxicology, 1999, Vol.122 (1), p.121-129 |
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Main Authors: | , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | This work was aimed at evaluating the gill carbonic anhydrase (CA) activity of the estuarine crab
Chasmagnathus granulata exposed in vivo to cadmium, at different salinities. The in vivo effect of the specific inhibitor acetazolamide (AZ) was also assayed. Besides, the inhibition of CA activity by different heavy metals (cadmium, copper, zinc) and AZ were evaluated under in vitro conditions. For the in vivo assays, adult males were acclimated to salinities of 2.5 or 30‰. The corresponding 96-h LC
50 of cadmium was 2.69 mg l
−1 at 2.5‰, and >50 mg l
−1 at 30‰. Cadmium only caused a significant lower CA activity than control at 2.5‰. EC
50 for CA inhibition was estimated to be 1.59 mg l
−1 at 2.5‰. Statistical differences in Na
+ hemolymphatic levels (
P |
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ISSN: | 0742-8413 1367-8280 |
DOI: | 10.1016/S0742-8413(98)10094-4 |