cDNA sequence and expression of a cold-responsive gene in Citrus unshiu

• Published in: Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 1999-02, Vol.63 (2), p.433-437
• Main Authors: Hara, M. (Shizuoka Univ. (Japan). Faculty of Agriculture), Wakasugi, Y, Ikoma, Y, Yano, M, Ogawa, K, Kuboi, T
• Format: Article
• Language: English
• Subjects: Adaptation to environment and cultivation conditions; Agronomy. Soil science and plant productions; Amino Acid Sequence; Base Sequence; Biological and medical sciences; Blotting, Northern; cDNA cloning; Citrus - chemistry; Citrus - genetics; CITRUS UNSHIU; COLD; cold stress; Cold Temperature; CuCOR19 protein; dehydrin family; DNA Probes - chemistry; DNA, Complementary - chemistry; DNA, Plant - chemistry; EXPRESION GENICA; EXPRESSION DES GENES; FRIO; FROID; Fundamental and applied biological sciences. Psychology; GENE EXPRESSION; Gene Expression Regulation, Plant; Genetics and breeding of economic plants; Molecular Sequence Data; Molecular Weight; NUCLEOTIDE SEQUENCE; Plant Proteins - chemistry; Plant Proteins - genetics; RESISTANCE A LA TEMPERATURE; RESISTENCIA A LA TEMPERATURA; RNA, Plant - chemistry; SECUENCIA NUCLEOTIDICA; Sequence Alignment; Sequence Homology, Amino Acid; SEQUENCE NUCLEOTIDIQUE; Surface Properties; TEMPERATURE RESISTANCE; Varietal selection. Specialized plant breeding, plant breeding aims
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1271/bbb.63.433

A cDNA clone encoding a protein (CuCOR19), the sequence of which is similar to Poncirus COR19, of the dehydrin family was isolated from the epicarp of Citrus unshiu. The molecular mass of the predicted protein was 18,980 daltons. CuCOR19 was highly hydrophilic and contained three repeating elements including Lys-rich motifs. The gene expression in leaves increased by cold stress