The Effect of TGF-beta Delivered Through a Collagen Scaffold on Wound Healing

In this preliminary study, the wound healing response of full-thickness skin defects to transforming growth factor beta (TGF-beta) incorporated in a collagen scaffold was evaluated. The objectives of this study were (1) to compare the effects of TGF-beta on the cellular and tissue response and mecha...

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Bibliographic Details
Published in:Journal of investigative surgery 1999, Vol.12 (2), p.89-100
Main Author: Abhay Pandit, Rajiv Ashar, Dale Feldman
Format: Article
Language:English
Subjects:
Animals
Bandages
Collagen
Drug Delivery
Transforming Growth Factor-beta
Wound
Healing
Epithelial Cells - cytology
Epithelial Cells - drug effects
Epithelial Cells - physiology
Inflammation
Polyurethanes
Rabbits
Skin - cytology
Skin - drug effects
Skin - pathology
Time Factors
Transforming Growth Factor beta - administration & dosage
Transforming Growth Factor beta - pharmacology
Wound Healing - drug effects
Wound Healing - physiology
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Summary:In this preliminary study, the wound healing response of full-thickness skin defects to transforming growth factor beta (TGF-beta) incorporated in a collagen scaffold was evaluated. The objectives of this study were (1) to compare the effects of TGF-beta on the cellular and tissue response and mechanical properties and (2) to determine the effects of a collagen scaffold on wound healing when compared to control. Three 3 3 cm, full-thickness defects were created on the dorsi of 15 New Zealand White rabbits. Each rabbit had a control (no treatment),collagen scaffold, and collagen scaffold with TGF-beta (2mug/cm2). All the wounds were covered with a transparent polyurethane dressing. There were three periods of study (1, 2, and 3 weeks) with five rabbits in each period. The volume fraction of the tissue and cells was histomorphometrically determined for each wound. A greater inflammatory response was found in the collagen scaffold-treated group,butthe fastestepithelialization and contraction rates were associated with TGF-beta and collagen. No significant differences in the mechanical strength between the different treatment groups were seen. Overall, TGF-beta delivered through a collagen scaffold enhanced the healing process and showed promise for future clinical applications.
ISSN:0894-1939
1521-0553
DOI:10.1080/089419399272647